Objective : To determine the relations between human papilloma viruses type 16 and type 18
infection and the expression of p53
protein in invasive
ductal carcinoma. Methods : We detected the expression of HPV 16/18
DNA and p53
protein in invasive
ductal carcinoma in 45 cases, breast
fibroadenoma in 20 cases and normal breast tissues in 20 cases. HPV detection was performed on
paraffin sections using
biotin-labeled probes by in situ hybridization and p53
protein expression was evaluated by immunohistochemistry. Results : The expression rate of HPV 16/18
DNA and p53
protein in invasive
ductal carcinoma is significantly higher than those in breast
fibroadenoma and normal breast tissues (p<0.05); the expression in cases with axillary
lymph node metastasis is dramatically higher than those without (p<0.05); the expression of p53
protein increases with TMN staging advance. The expression of HPV16/18
DNA was significantly correlated with the expression of p53
protein (p<0.05). Conclusion : Both HPV16/18
infection and p53 mutation participate the occurrence and progress of invasive
ductal carcinoma, and HPV 16/18
infection may be the major factor to cause p53 mutation.