In acute experiments on the rats with cannulated common biliary duct, the influence ofamylin on the level of bile secretion and
bile acids spectrum was investigated. It was shown that subcutaneous administration of
amylin at the dose 1 mg/kg
body weight doesn't affect the volume of secreted bile. Under these conditions, the concentration of
taurocholic acid was increased and the concentration of tauroconjugates of chenodeoxycholic and deoxycholic
acids was decreased in the bile. At the same time, the concentration of glycocholates remained constant and of unconjugated
bile acids was decreased. This redistribution of
bile acids spectrum leads to an increase in the coefficient of conjugation.
Amylin changes the ratio of trygydroxy- and dygydroxycholates in secreted bile leading to an increase in the coefficient of hydroxylation. These results suggest that
amylin enhances the processes of conjugation and hydroxylation of
bile acids in hepatocytes that results in improvement of
detergent properties of the bile, particularly, the ability of the bile to maintain the
cholesterol in dissolved state. At the lowest effective dose,
amylin does not alter the concentration of
glucose in the blood.