Abstract | IMPORTANCE: OBJECTIVE: To investigate the ciliary epithelium as a possible source of VEGF in human eyes enucleated for intractable neovascular glaucoma. DESIGN, SETTING, AND PARTICIPANTS: In this proof-of-concept, laboratory-based study, 16 human enucleated eyes (8 with neovascular glaucoma and 8 as controls) were investigated. MAIN OUTCOMES AND MEASURES: RESULTS: In eyes with neovascular glaucoma, strong VEGF immunoreaction in the nonpigmented epithelial cells of the ciliary processes and in the retina was noted. In situ hybridization for VEGF messenger RNA revealed a similar pattern, with positive stain results only in eyes with neovascular glaucoma. A minimal amount of VEGF immunostaining was seen in control eyes. CONCLUSIONS AND RELEVANCE: The nonpigmented ciliary epithelium is an important site of VEGF synthesis in patients with neovascular glaucoma. The ciliary epithelium may represent an additional focus of treatment in the management of neovascular glaucoma, especially in eyes that are nonresponsive to panretinal photocoagulation.
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Authors | Kakarla V Chalam, Vikram S Brar, Ravi K Murthy |
Journal | JAMA ophthalmology
(JAMA Ophthalmol)
Vol. 132
Issue 11
Pg. 1350-4
(Nov 2014)
ISSN: 2168-6173 [Electronic] United States |
PMID | 25079256
(Publication Type: Journal Article)
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Chemical References |
- RNA, Messenger
- VEGFA protein, human
- Vascular Endothelial Growth Factor A
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Topics |
- Ciliary Body
(metabolism)
- Epithelium
(metabolism)
- Eye Enucleation
- Gene Expression
- Glaucoma, Neovascular
(metabolism, surgery)
- Humans
- Immunoenzyme Techniques
- In Situ Hybridization
- RNA, Messenger
(genetics)
- Vascular Endothelial Growth Factor A
(genetics, metabolism)
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