[Experimental study on the protective effect of anisodamine on flaps with ischemia reperfusion injury].

Abstract

OBJECTIVE:

To observe the effect of anisodamine (Ani) injection on the survival rate and histologic result of flaps with ischemia-reperfusion injury, so as to demonstrate the protective effect of Ani on the flap survival.

METHODS:

A total of 48 healthy male Wistar rats were randomly divided into model control, normal saline(NS) and anisodamine groups, with 16 rats in each group. An 3 cm x 6 cm axial flap was formed at the right lower abdomen with abdominal superficial blood vessel as the pedicle. 0.5 cm x 0.5 cm skin tissue was taken from the middle part of flaps in each group, immediately after operation, 12, 18, 24 h after operation. The superoxide dismutase (SOD), nitric oxide (NO), nuclear factor-kappaB contents in the specimens were detected. The histologic study was also performed. The flap survival rate was recorded 7 days after operation.

RESULTS:

Flap survival rate was (78.6 +/- 7.3) % in Anisodamine group. 12, 18, 24 h after reperfusion injury, the SOD was (103.3 +/- 3.9), (82.6 +/- 3.8), (67.5 +/- 4.6) U/mg; the NO was (5.33 +/- 2.05), (4.75 +/- 1.68), (4.15 +/-1.59) nmol/mg; the NF-kappaB was 0.211 +/- 0.039, 0.313 +/- 0.033, 0.096 +/- 0.028. The contents of SOD, NO and NF-kappaB had the statistical difference of at different time. The skin pathological changes in Anisodamine group was obviously better than those in NS group. Flap survival rate in Anisodamine group was significantly higher than that in NS group.

CONCLUSIONS:

In the flap with ischemia-reperfusion injury, Anisodamine can reduce the damage of free radical, increase the blood flow, reduce the production of NF-KB, decrease inflammatory reaction. So Anisodamine can increase the survival rate of flaps with ischemia reperfusion injury.

• Authors: Qiang Lu, Ying-Biao Chen, Fan Dai, Shu-Lin Qiu
• Journal: Zhonghua zheng xing wai ke za zhi = Zhonghua zhengxing waike zazhi = Chinese journal of plastic surgery (Zhonghua Zheng Xing Wai Ke Za Zhi) Vol. 29 Issue 3 Pg. 202-5 (May 2013) ISSN: 1009-4598 [Print] China
• PMID: 25069348 (Publication Type: Journal Article)

Chemical References

• NF-kappa B
• Solanaceous Alkaloids
• Vasodilator Agents
• anisodamine
• Nitric Oxide
• Superoxide Dismutase

Topics

• Animals
• Graft Survival (drug effects)
• Male
• NF-kappa B (analysis)
• Nitric Oxide (analysis)
• Random Allocation
• Rats
• Rats, Wistar
• Reperfusion Injury (prevention & control)
• Solanaceous Alkaloids (therapeutic use)
• Superoxide Dismutase (analysis)
• Surgical Flaps (blood supply, pathology)
• Vasodilator Agents (therapeutic use)