Siegesbeckia glabrescens attenuates allergic airway inflammation in LPS-stimulated RAW 264.7 cells and OVA induced asthma murine model.

Abstract

Siegesbeckia glabrescens (SG) is a plant growing in Korea that is used as a traditional medicine for various inflammatory diseases. In this study, we investigated the protective effects of SG extract on allergic asthma in an ovalbumin (OVA)-induced asthma murine model and lipopolysaccharide (LPS)-stimulated RAW264.7 cells. Female BALB/c mice were sensitized by intraperitoneal injection of OVA on days 0 and 14 and then challenged with OVA from days 21 to 23. SG (30mg/kg) was administered by oral gavage 1h before the OVA challenge. LPS-stimulated RAW264.7 cells were evaluated to determine their levels of nitric oxide (NO). The SG significantly reduced the number of inflammatory cells in bronchoalveolar lavage (BAL) fluid and also reduced IL-4, IL-5, IL-13, eotaxin and immunoglobulin E in OVA-sensitized/challenged mice. SG also effectively reduced airway inflammation and mucus overproduction in lung tissue in addition to decreasing the expression of iNOS and COX-2. In LPS-stimulated RAW264.7 cells, SG treatment significantly reduced the levels of NO. These findings indicate that SG effectively suppressed inflammatory responses, and its effects appear to be related to reduction in iNOS and COX-2 expression. Therefore, we suggest that SG may have potential use as a therapeutic agent for inflammatory diseases such as allergic asthma.

• Authors: Chan-Mi Jeon, In-Sik Shin, Na-Rae Shin, Ju-Mi Hong, Ok-Kyoung Kwon, Hui-Seong Kim, Sei-Ryang Oh, Pyung-Keun Myung, Kyung-Seop Ahn
• Journal: International immunopharmacology (Int Immunopharmacol) Vol. 22 Issue 2 Pg. 414-9 (Oct 2014) ISSN: 1878-1705 [Electronic] Netherlands
• PMID: 25066761 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier B.V. All rights reserved.

Chemical References

• Anti-Inflammatory Agents
• Cytokines
• Lipopolysaccharides
• Plant Preparations
• Nitric Oxide
• Immunoglobulin E
• Ovalbumin
• Nitric Oxide Synthase Type II
• Nos2 protein, mouse
• Ptgs2 protein, mouse
• Cyclooxygenase 2

Topics

• Animals
• Anti-Inflammatory Agents (pharmacology, therapeutic use)
• Asteraceae
• Asthma (drug therapy, immunology, metabolism)
• Bronchoalveolar Lavage Fluid (cytology, immunology)
• Cell Count
• Cell Line
• Cell Survival (drug effects)
• Cyclooxygenase 2 (metabolism)
• Cytokines (immunology)
• Disease Models, Animal
• Female
• Immunoglobulin E (blood)
• Lipopolysaccharides
• Lung (drug effects, immunology, metabolism)
• Mice
• Mice, Inbred BALB C
• Mucus (metabolism)
• Nitric Oxide (metabolism)
• Nitric Oxide Synthase Type II (metabolism)
• Ovalbumin (immunology)
• Phytotherapy
• Plant Preparations (pharmacology, therapeutic use)