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An H1-H3 chimeric influenza virosome confers complete protection against lethal challenge with PR8 (H1N1) and X47 (H3N2) viruses in mice.

Abstract
Annual health threats and economic damages caused by influenza virus are still a main concern of the World Health Organization and other health departments all over the world. An influenza virosome is a highly efficient immunomodulating carrier mimicking the natural antigen presentation pathway and has shown an excellent tolerability profile due to its biocompatibility and purity. The major purpose of this study was to construct a new chimeric virosome influenza vaccine containing hemagglutinin (HA) and neuraminidase (NA) proteins derived from the A/PR/8/1934 (H1N1) (PR8) and A/X/47 (H3N2) (X47) viruses, and to evaluate its efficacy as a vaccine candidate in mice. A single intramuscular vaccination with the chimeric virosomes provided complete protection against lethal challenge with the PR8 and X47 viruses. The chimeric virosomes induced high IgG antibody responses as well as hemagglutination inhibition (HAI) titers. HAI titers following the chimeric virosome vaccination were at the same level as the whole inactivated influenza vaccine. Mice immunized with the chimeric virosomes displayed considerably less weight loss and exhibited significantly reduced viral load in their lungs compared with the controls. The chimeric virosomes can be used as an innovative vaccine formulation to confer protection against a broad range of influenza viruses.
AuthorsAsghar Abdoli, Hoorieh Soleimanjahi, Masoumeh Tavassoti Kheiri, Abbas Jamali, Vahideh Mazaheri, Meghdad Abdollahpour Alitappeh
JournalPathogens and disease (Pathog Dis) Vol. 72 Issue 3 Pg. 197-207 (Dec 2014) ISSN: 2049-632X [Electronic] United States
PMID25066138 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Copyright© 2014 Federation of European Microbiological Societies. Published by John Wiley & Sons Ltd. All rights reserved.
Chemical References
  • Antibodies, Viral
  • Hemagglutinin Glycoproteins, Influenza Virus
  • Immunoglobulin G
  • Influenza Vaccines
  • Recombinant Fusion Proteins
  • Vaccines, Synthetic
  • Virosomes
Topics
  • Animals
  • Antibodies, Viral (blood)
  • Cell Line
  • Dogs
  • Female
  • Hemagglutination Inhibition Tests
  • Hemagglutinin Glycoproteins, Influenza Virus (genetics, immunology)
  • Host-Pathogen Interactions (genetics, immunology)
  • Immunoglobulin G (blood)
  • Influenza A Virus, H1N1 Subtype (genetics, immunology, pathogenicity)
  • Influenza A Virus, H3N2 Subtype (genetics, immunology, pathogenicity)
  • Influenza Vaccines (genetics, immunology)
  • Lung (immunology, virology)
  • Mice
  • Mice, Inbred BALB C
  • Orthomyxoviridae Infections (immunology, prevention & control, virology)
  • Recombinant Fusion Proteins (genetics, immunology)
  • Vaccines, Synthetic (genetics, immunology)
  • Virosomes (genetics, immunology)

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