In vitro photodynamic inactivation of Sporothrix schenckii complex species.

Abstract

Photodynamic therapy has been applied successfully against cutaneous and subcutaneous mycoses. We applied methylene blue as a photosensitizing agent and light emitting diode (InGaAlP) against Sporothrix schenckii complex species in an in vitro assay. The viability of the conidia was determined by counting colony-forming units. Methylene blue in conjunction with laser irradiation was able to inhibit the growth of all tested samples. The in vitro inhibition of Sporothrix spp. isolates by laser light deserves in vivo experimental and clinical studies since it may be a promising treatment for cutaneous and subcutaneous sporotrichosis.

• Authors: Débora Alves Nunes Mario, Laura Bedin Denardi, Daniela Isabel Brayer Pereira, Janio Morais Santurio, Sydney Hartz Alves
• Journal: Medical mycology (Med Mycol) Vol. 52 Issue 7 Pg. 770-3 (Oct 2014) ISSN: 1460-2709 [Electronic] England
• PMID: 25049038 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © The Author 2014. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology. All rights reserved. For permissions, please e-mail: [email protected].

Chemical References

• Photosensitizing Agents
• Methylene Blue

Topics

• Colony Count, Microbial
• Disinfection (methods)
• Humans
• Lasers
• Methylene Blue (metabolism)
• Microbial Viability (drug effects, radiation effects)
• Photochemotherapy (methods)
• Photosensitizing Agents (metabolism)
• Spores, Fungal (drug effects, radiation effects)
• Sporothrix (drug effects, radiation effects)