[Integrins mediate the migration of HepG2 cells induced by low shear stress].

Abstract

Low shear stress is a component of the tumor microenvironment in vivo and plays a key role in regulating cancer cell migration and invasion. The integrin, as a mechano-sensors mediating and integrating mechanical and chemical signals, induce the adhesion between cells and extracellular matrix (ECM). The purpose of this study is to investigate the effect of low shear stress (1.4 dyn/cm2)on the migration of HepG2 cells and the expression of integrin. Scratch wound migration assay was performed to examine the effect of low shear stress on the migration of HepG2 cells at 0 h, 1 h, 2 h and 4 h, respectively. F-actin staining was used to detect the expression of F-actin in HepG2 cells treated with low shear stress at 2 h and 4 h. Western blot analysis was carried out to determine the effect of low shear stress on the expression of integrin at different durations. The results showed that the migrated distance of HepG2 cells and the expression of F-actin increased significantly compared with the controls. The integrin alpha subunits showed a different time-dependent expression, suggesting that various subunits of integrin exhibit different effects in low shear stress regulating cancer cells migration.

• Authors: Wang Lijuan, Xiaoheng Liu, Hongchi Yu, Fating Zhou, Huilin Chen, Qianqi Liu
• Journal: Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi (Sheng Wu Yi Xue Gong Cheng Xue Za Zhi) Vol. 31 Issue 2 Pg. 336-40 (Apr 2014) ISSN: 1001-5515 [Print] China
• PMID: 25039138 (Publication Type: Journal Article)

Chemical References

• Actins
• Integrins

Topics

• Actins (physiology)
• Cell Movement
• Extracellular Matrix (physiology)
• Hep G2 Cells
• Humans
• Integrins (physiology)
• Stress, Mechanical