Antitumor metastasis activity of pectic polysaccharide purified from the peels of Korean Citrus Hallabong.

Abstract

A polysaccharide fraction, HBE-III, was successfully purified in a high yield (40.4%) from its crude polysaccharide (HBE-0) which was prepared from pectinase hydrolysates of the peels of the Korean Citrus Hallabong. In experimental lung metastasis studies of Colon 26-M3.1 carcinoma cells, prophylactic administration of HBE-III significantly inhibited lung metastasis in a dose-dependent manner. In an in vitro cytotoxicity analysis, HBE-III (up to 1000 μg/mL) did not affect the growth of Colon 26-M3.1 cells and normal cells. HBE-III enhanced production of IL-6 and IL-12 by murine peritoneal macrophages. In an assay for natural killer (NK) cell activity, HBE-III (1000 μg/mouse, i.v.) significantly augmented NK cytotoxicity against Yac-1 tumor cells. The depletion of NK cells by injection of mouse anti-asialo GM1 serum abolished the inhibitory effect of HBE-III on lung metastasis of Colon 26-M3.1 cells. These data suggest that HBE-III may inhibit tumor metastasis via activation of macrophages and NK cells.

• Authors: Eun Hye Lee, Hye-Ryung Park, Myoung-Sook Shin, Sun Young Cho, Hyuk-Joon Choi, Kwang-Soon Shin
• Journal: Carbohydrate polymers (Carbohydr Polym) Vol. 111 Pg. 72-9 (Oct 13 2014) ISSN: 1879-1344 [Electronic] England
• PMID: 25037331 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier Ltd. All rights reserved.

Chemical References

• Antineoplastic Agents, Phytogenic
• Interleukin-6
• Polysaccharides
• rhamnogalacturonan II
• Interleukin-12
• Pectins

Topics

• Animals
• Antineoplastic Agents, Phytogenic (chemistry, isolation & purification, therapeutic use)
• Cell Line
• Cell Line, Tumor
• Citrus (chemistry)
• Colon (immunology, pathology)
• Colonic Neoplasms (immunology, pathology)
• Female
• Hydrolysis
• Interleukin-12 (immunology)
• Interleukin-6 (immunology)
• Killer Cells, Natural (drug effects, immunology, pathology)
• Lung (drug effects, immunology, pathology)
• Lung Neoplasms (immunology, pathology, prevention & control, secondary)
• Mice
• Mice, Inbred BALB C
• Neoplasm Metastasis (immunology, pathology, prevention & control)
• Pectins (chemistry, isolation & purification, therapeutic use)
• Polysaccharides (chemistry, isolation & purification, therapeutic use)