Abstract |
Thioredoxin system is an attractive target to overcome radioresistance in cancer therapy. The redox enzyme thioredoxin reductase (TrxR) plays a vital role in restoring cellular thiol redox balance disrupted by radiation-induced reactive oxygens species (ROS) generation and oxidative damage. In this study, a series of 1,2,5-selenadiazoles have been synthesized and identified as highly effective inhibitors of TrxR to disrupt the intracellular redox balance, and thus significantly enhanced the sensitivity of cancer cells to X-ray. Upon irradiation, 1,2,5-selenadiazoles displayed a marked synergistic inhibitory effect on radioresistant A375 melanoma cell through enhancement of ROS overproduction, and subsequent induction of ROS-promoted apoptotic pathways, which triggered then mitochondrial dysfunction and caspase activation, finally resulted in augment of radiotherapeutic efficacy. Interestingly, we also found the interaction sites between 1,2,5-selenadiazole and the model peptide of TrxR, which can be confirmed by MALDI-ToF-MS. These results clearly demonstrate TrxR as a potential target for therapy of radioresistant cancers, and selenadiazole derivatives may be attractive radiosensitizing agent by targeting TrxR.
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Authors | Yuan-Wei Liang, Junsheng Zheng, Xiaoling Li, Wenjie Zheng, Tianfeng Chen |
Journal | European journal of medicinal chemistry
(Eur J Med Chem)
Vol. 84
Pg. 335-42
(Sep 12 2014)
ISSN: 1768-3254 [Electronic] France |
PMID | 25036792
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2014 Elsevier Masson SAS. All rights reserved. |
Chemical References |
- Azoles
- Enzyme Inhibitors
- Organoselenium Compounds
- Radiation-Sensitizing Agents
- Reactive Oxygen Species
- Thioredoxin-Disulfide Reductase
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Topics |
- Apoptosis
(drug effects)
- Azoles
(chemical synthesis, chemistry, pharmacology)
- Cell Survival
(drug effects)
- Dose-Response Relationship, Drug
- Enzyme Inhibitors
(chemical synthesis, chemistry, pharmacology)
- Gamma Rays
- Humans
- Molecular Structure
- Neoplasms
(drug therapy, enzymology, pathology, radiotherapy)
- Organoselenium Compounds
(chemical synthesis, chemistry, pharmacology)
- Radiation Tolerance
(drug effects)
- Radiation-Sensitizing Agents
(chemical synthesis, chemistry, pharmacology)
- Reactive Oxygen Species
(metabolism)
- Structure-Activity Relationship
- Thioredoxin-Disulfide Reductase
(antagonists & inhibitors, metabolism)
- Tumor Cells, Cultured
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