Enhancing lung cancer diagnosis: electrochemical simultaneous bianalyte immunosensing using carbon nanotubes-chitosan nanocomposite.

Abstract

A label-free electrochemical bianalyte immunosensor has been designed for simultaneous detection of lung cancer biomarkers (anti-MAGE A2 and anti-MAGE A11) using carbon nanotubes-chitosan (CNT-CHI) composite. To achieve this, acid-functionalized single-walled CNTs were used to prepare CNT-CHI gel and electrodes were fabricated by drop casting method onto graphite surface. Lung cancer biomarkers specific antigens (Ag), i.e., MAGE A2 and MAGE A11, were covalently immobilized onto CNT-CHI/graphite electrode separately for fabrication process. Fabricated immunoelectrodes (MAGE A2/CNT-CHI/graphite and MAGE A11/CNT-CHI/graphite) were characterized at each modification step by cyclic voltammetry (CV), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM). Both immunoelectrodes showed successful detection of respective analytes (anti-MAGE A2 and anti-MAGE A11) from 5 fg mL(-1) to 50 ng mL(-1) using differential pulse voltammetry (DPV). Both Ag/CNT-CHI/graphite immunoelectrodes (using MAGE A2 and MAGE A11) were independently capable of distinguishing specific and nonspecific analytes like CD59, D-dimers, etc. Response studies of both immunoelectrodes revealed successful demonstration of simultaneous detection of anti-MAGE A2 and A11 independently in a single experimental run when exposed to a mixture of various analyte concentrations in different combinations irrespective of the presence of other analyte present in the same vessel.

• Authors: Meenakshi Choudhary, Anu Singh, Satbir Kaur, Kavita Arora
• Journal: Applied biochemistry and biotechnology (Appl Biochem Biotechnol) Vol. 174 Issue 3 Pg. 1188-200 (Oct 2014) ISSN: 1559-0291 [Electronic] United States
• PMID: 25024132 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Neoplasm
• MAGEA11 protein, human
• Nanotubes, Carbon
• Neoplasm Proteins
• Graphite
• Chitosan

Topics

• Antigens, Neoplasm (immunology, isolation & purification)
• Biosensing Techniques
• Chitosan (chemistry)
• Graphite (chemistry)
• Humans
• Lung Neoplasms (diagnosis, immunology)
• Microscopy, Electron, Scanning
• Nanocomposites (chemistry)
• Nanotubes, Carbon (chemistry)
• Neoplasm Proteins (immunology, isolation & purification)
• Spectroscopy, Fourier Transform Infrared