TGF-β detection and measurement in murine B cells: pros and cons of the different techniques.

Abstract

Recent studies have demonstrated the importance of regulatory B cells in autoimmune, allergic, and inflammatory diseases. These B cells have an ability to suppress excessive immune reactions by multiple mechanisms. Most studies have focused on IL-10-producing B cells, but we have previously reported that transforming growth factor (TGF)-β1 secretion by B cells also plays an important role in intestinal homeostasis and mucosal inflammation. B cell-secreted TGF-β may be involved in the regulation of T cell immunity (differentiation, proliferation, and apoptosis) that is relevant to the pathogenesis of autoimmune or inflammatory disease. Here, we provide detailed instruction for detecting and measuring TGF-β produced by B cells.

• Authors: Yoshiyuki Mishima, Shunji Ishihara, Jonathan J Hansen, Yoshikazu Kinoshita
• Journal: Methods in molecular biology (Clifton, N.J.) (Methods Mol Biol) Vol. 1190 Pg. 71-80 ( 2014) ISSN: 1940-6029 [Electronic] United States
• PMID: 25015274 (Publication Type: Journal Article)

Chemical References

• RNA, Messenger
• Transforming Growth Factor beta

Topics

• Animals
• B-Lymphocytes (cytology, immunology, metabolism)
• Cell Culture Techniques (methods)
• Cell Separation (methods)
• Enzyme-Linked Immunosorbent Assay (methods)
• Flow Cytometry (methods)
• Mice
• Mice, Inbred C57BL
• RNA, Messenger (analysis, genetics)
• Real-Time Polymerase Chain Reaction (methods)
• Transforming Growth Factor beta (analysis, genetics, immunology)