hesperidin induces paraptosis like cell death in hepatoblastoma, HepG2 Cells: involvement of ERK1/2 MAPK [corrected].

Abstract

Hesperidin, a natural flavonoid abundantly present in Citrus is known for its anti-cancer, anti-oxidant and anti-inflammatory properties. In this study we examined the effect of hesperidin on HepG2 cells. HepG2 cells treated with various concentration of hesperidin undergo a distinct type of programed cell death. Cytoplasmic vacuolization, mitochondria and endoplasmic reticulum swelling and uncondensed chromatin were observed in hesperidin treated cells. DNA electrophoresis show lack of DNA fragmentation and western blot analysis demonstrates lack of caspase activation and PARP cleavage. It was observed that hesperidin induced cell death is nonautophagic and also activate mitogen activated protein kinase ERK1/2. Taken together, the data indicate that hesperidin induces paraptosis like cell death in HepG2 cells with the activation of ERK1/2. Thus our finding suggests that hesperidin inducing paraptosis may offer an alternative tool in human liver carcinoma therapy.

• Authors: Silvia Yumnam, Hyeon Soo Park, Mun Ki Kim, Arulkumar Nagappan, Gyeong Eun Hong, Ho Jeong Lee, Won Sup Lee, Eun Hee Kim, Jae Hyeon Cho, Sung Chul Shin, Gon Sup Kim
• Journal: PloS one (PLoS One) Vol. 9 Issue 6 Pg. e101321 ( 2014) ISSN: 1932-6203 [Electronic] United States
• PMID: 24977707 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Hesperidin
• Mitogen-Activated Protein Kinase 1
• Mitogen-Activated Protein Kinase 3

Topics

• Antineoplastic Agents (pharmacology)
• Cell Death
• Endoplasmic Reticulum (drug effects)
• Hep G2 Cells
• Hesperidin (pharmacology)
• Humans
• Mitochondria (drug effects)
• Mitogen-Activated Protein Kinase 1 (metabolism)
• Mitogen-Activated Protein Kinase 3 (metabolism)