Non-aggregated protamine-coated poly(lactide-co-glycolide) nanoparticles of cisplatin crossed blood-brain barrier, enhanced drug delivery and improved therapeutic index in glioblastoma cells: in vitro studies.

Abstract

BACKGROUND AND OBJECTIVES:

Non-aggregated protamine impregnated poly(lactide-co-glycolide) nanoparticles of cisplatin (Pt-PLGA NPs) were synthesized to augment brain delivery.

METHODS AND RESULTS:

The mean particle size of Pt-PLGA NPs and PLGA NPs were observed to be 173.2 ± 7.9 nm and 140 ± 10.2 nm, respectively. The Pt-PLGA NPs significantly (p < 0.05, one-way analysis of variance; ANOVA) delivered higher amount (172.41 ± 15.04 μg) of cisplatin in comparison to 110.48 ± 4.71 μg by PLGA NPs and 20.83 ± 1.65 μg by cisplatin solution across in vitro bovine brain microvessel endothelial cells. Cisplatin bearing Pt-PLGA NPs was found to be highly cytotoxic to U87 glioblastoma cells with an IC50 of 2.1 μM as compared (one-way ANOVA, p < 0.05) to PLGA NPs (3.9 μM) and cisplatin alone (13.33 μM). Impregnation with Pt enhanced the uptake of PLGA NPs in U87 glioblastoma cells as compared to PLGA NPs by following endocytosis mechanism.

CONCLUSION:

Cisplatin-loaded Pt-PLGA NPs compel preclinical tumour regression study to further improve its utility against glioblastoma.

• Authors: Neel Kamal Dhami, Ravi Shankar Pandey, Upendra Kumar Jain, Ramesh Chandra, Jitender Madan
• Journal: Journal of microencapsulation (J Microencapsul) Vol. 31 Issue 7 Pg. 685-93 ( 2014) ISSN: 1464-5246 [Electronic] England
• PMID: 24963955 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents
• Drug Carriers
• Protamines
• Polyglactin 910
• Cisplatin

Topics

• Animals
• Antineoplastic Agents (chemistry, pharmacokinetics, pharmacology)
• Blood-Brain Barrier (metabolism, pathology)
• Brain Neoplasms (drug therapy, metabolism, pathology)
• Cattle
• Cell Line, Tumor
• Cisplatin
• Drug Carriers (chemistry, pharmacokinetics, pharmacology)
• Glioblastoma (drug therapy, metabolism, pathology)
• Humans
• Nanoparticles (chemistry)
• Polyglactin 910 (chemistry, pharmacokinetics, pharmacology)
• Protamines (chemistry, pharmacokinetics, pharmacology)