[Effect of sodium tanshinone II (A) sulfonate on Ang II -induced atrial fibroblast collagen synthesis and TGF-beta1 activation].

Abstract

OBJECTIVE:

To observe the effect of sodium tanshinone II (A) sulfonate (STS) on Ang II -induced atrial fibroblast collagen synthesis and TGF-beta1 activation.

METHOD:

Atrial fibroblasts of neonatal rats were cultured to determine the content of collagen protein. The original synthesis rate determined by the [3H]-proline incorporation method was taken as the index for myocardial fibrosis. The content of active TGF-beta1 and total TGF-beta1 in cell culture supernatants were tested and cultured by ELISA. The expression of thrombospondin-1 (TSP-1) was assessed by using Western blot.

RESULT:

Ang II could significantly increase the content of atrial fibroblast collagen and the collagen synthesis rate, the TSP-1 expression and the concentration of active TGF-beta1, without any obvious change in total TGF-beta1. After the STS treatment, all of the indexes, apart from total TGF-beta1, were obviously down-regulated.

CONCLUSION:

STS could decrease the secretion of Ang II -induced atrial fibroblast collagen and the synthesis rate. Its mechanism is related to the inhibition of TSP-1/TGF-beta1 pathway.

• Authors: Le Yang, Xiao-Jing Zou, Zhao Yin, Hong-Zhen Hao
• Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (Zhongguo Zhong Yao Za Zhi) Vol. 39 Issue 6 Pg. 1093-6 (Mar 2014) ISSN: 1001-5302 [Print] China
• PMID: 24956857 (Publication Type: English Abstract, Journal Article)

Chemical References

• Phenanthrenes
• Thrombospondin 1
• Transforming Growth Factor beta1
• Angiotensin II
• tanshinone II A sodium sulfonate
• Collagen

Topics

• Angiotensin II (pharmacology)
• Animals
• Collagen (biosynthesis)
• Fibroblasts (cytology, drug effects, metabolism)
• Gene Expression Regulation (drug effects)
• Heart Atria (cytology)
• Phenanthrenes (pharmacology)
• Rats
• Rats, Wistar
• Signal Transduction (drug effects)
• Thrombospondin 1 (metabolism)
• Transforming Growth Factor beta1 (metabolism)