[Intervention effect of Dachengqi Granule on apoptosis of small intestine smooth muscle cells in rats with multiple organ dysfunction syndrome].

Abstract

OBJECTIVE:

To observe the intervention of Dachengqi Granule (DG) on the apoptosis of small intestine smooth muscle cells (SMCs) in rats with multiple organ dysfunction syndrome (MODS) and its mechanisms.

METHODS:

Healthy 100 adult Wistar rats were randomly divided into the control group (n =20), the MODS model group (n =40), and the DG group (n =40).E. coli suspension was peritoneally injected to rats in the model group and the DG group to establish bacterial peritonitis induced MODS model. DG at 1 mL/100 g was administered by gastrogavage to rats of the DG group, twice daily for 3 successive days. Twenty-four hours after modeling, the proximal segment of intestine was taken and stained by using terminal-deoxynucleotidyl transferase mediated nick end labeling (TUNEL) and immunohistochemistry. Changes of apoptosis quantity of SMCs and the expression of Bcl-2 associated X protein (Bax), B cell lymphoma/leukemia-2 (Bcl-2) and cytochrome c protein (Cyt c) in mitochondrial apoptotic signaling pathway were observed.

RESULTS:

Compared with the control group, the apoptosis quantity of SMCs and the expression of Bax and Cyt c protein significantly increased, and the expression of Bcl-2 protein significantly decreased in the MODS model group (P <0.01). Compared with the MODS model group, the apoptosis quantity of SMCs and the expression of Bax and Cyt c proteins significantly decreased, and the expression of Bcl-2 protein significantly increased in the DG group (allP <0.01).

CONCLUSION:

DG could inhibit apoptosis of SMCs through suppressing activation of mitochondrial apoptotic signaling pathway in intestinal SMCs, thus promoting the recovery of the gastrointestinal motility function in rats with MODS.

• Authors: Ming-Zheng Xie, Qing-Hui Qi
• Journal: Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine (Zhongguo Zhong Xi Yi Jie He Za Zhi) Vol. 34 Issue 5 Pg. 587-91 (May 2014) ISSN: 1003-5370 [Print] China
• PMID: 24941849 (Publication Type: Journal Article)

Chemical References

• Drugs, Chinese Herbal
• Plant Extracts
• Proto-Oncogene Proteins c-bcl-2
• bcl-2-Associated X Protein
• dachengqi decoction

Topics

• Animals
• Apoptosis (drug effects)
• Drugs, Chinese Herbal (pharmacology, therapeutic use)
• In Situ Nick-End Labeling
• Intestine, Small (physiopathology)
• Multiple Organ Failure (drug therapy)
• Muscle, Smooth (physiopathology)
• Myocytes, Smooth Muscle (drug effects)
• Plant Extracts (pharmacology, therapeutic use)
• Proto-Oncogene Proteins c-bcl-2
• Rats
• Rats, Wistar
• bcl-2-Associated X Protein (metabolism)