[Triptolide inhibits the inflammatory response of monocytes from rheumatoid arthritis patients by regulating miR-155].

Abstract

OBJECTIVE:

To explore the anti-inflammatory effect of triptolide (TPT) by regulating miR-155 in monocytes pre-stimulated by lipopolysaccharide (LPS) from rheumatoid arthritis (RA) patients.

METHODS:

Monocytes were isolated by CD14⁺ magnetic beads from peripheral blood mononuclear cells (PBMCs) of RA and stimulated by LPS for 24 hours. The levels of tumor-necrosis factor-α (TNF-α) and interleukin 6 (IL-6) in monocytes were detected by ELISA and the expression of miR-155 was measured by real-time quantitative PCR (qRT-PCR) in monocytes before and after the treatment of TPT at different concentrations. MiR-155 mimic and negative control were respectively transfected into the LPS-stimulated monocytes by Lipofectamine(TM)2000. Twenty-four hours later, the monocytes were treated with or without TPT for another 24 hours. TNF-α and IL-6 expressions in the cell culture supernatants were detected by ELISA and the expressions of suppressor of cytokine signaling-1 (SOCS1) and Src homology 2 domain-containing inositol 5-phosphatase 1 (SHIP-1) were tested by Western blotting.

RESULTS:

TPT suppressed the expressions of TNF-α, IL-6 and miR-155 in LPS-stimulated peripheral blood monocytes from RA patients. Over-expression of miR-155 significantly reversed the down-regulation of TNF-α and IL-6 by TPT in monocytes. TPT up-regulated the expressions of SOCS1 and SHIP-1 in monocytes, but over-expressed miR-155 antagonized the effect of TPT on SHIP-1 while the expression of SOCS1 was not affected.

CONCLUSION:

TPT suppressed the expression of miR-155 and up-regulated the release of SHIP-1, thus inhibiting the inflammatory response in the LPS-stimulated monocytes of RA patients.

• Authors: Anping Peng, Xianzhang Huang, Ruiping Liu, Xiaoyun Wang, Junhua Zhuang
• Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi) Vol. 30 Issue 6 Pg. 635-8 (Jun 2014) ISSN: 1007-8738 [Print] China
• PMID: 24909288 (Publication Type: English Abstract, Journal Article)

Chemical References

• Anti-Inflammatory Agents, Non-Steroidal
• Diterpenes
• Epoxy Compounds
• Interleukin-6
• Lipopolysaccharides
• MIRN155 microRNA, human
• MicroRNAs
• Phenanthrenes
• SOCS1 protein, human
• Suppressor of Cytokine Signaling Proteins
• Tumor Necrosis Factor-alpha
• triptolide
• Phosphoric Monoester Hydrolases
• inositol-polyphosphate 5-phosphatase

Topics

• Anti-Inflammatory Agents, Non-Steroidal (pharmacology)
• Arthritis, Rheumatoid (blood, genetics, pathology)
• Blotting, Western
• Cells, Cultured
• Diterpenes (pharmacology)
• Enzyme-Linked Immunosorbent Assay
• Epoxy Compounds (pharmacology)
• Gene Expression Regulation (drug effects)
• Humans
• Interleukin-6 (metabolism)
• Lipopolysaccharides (pharmacology)
• MicroRNAs (genetics)
• Monocytes (drug effects, metabolism, pathology)
• Phenanthrenes (pharmacology)
• Phosphoric Monoester Hydrolases (metabolism)
• Reverse Transcriptase Polymerase Chain Reaction
• Suppressor of Cytokine Signaling Proteins (metabolism)
• Tripterygium (chemistry)
• Tumor Necrosis Factor-alpha (metabolism)