Production and characterization of monoclonal antibodies against horse immunoglobulins useful for the diagnosis of equine diseases.

Abstract

Monoclonal antibodies (MAbs) against horse IgG were produced by immunizing Balb/c mice with purified horse IgG and were characterized in indirect ELISA versus purified immunoglobulins from donkey, cow, buffalo, sheep, pig, and chicken. Three MAbs (1B10B6C9, 1B10B6C10, 1B10B6E9) reacted only with horse and donkey IgG and IgM and, in western blotting, were specific for the Fc fragment of equine IgG. MAb 1B10B6E9 was used in chemiluminescent immunoblotting assay for the diagnosis of dourine and in indirect immunofluorescence assay (IFA) for the diagnosis of African horse sickness and dourine.

• Authors: Tiziana Di Febo, Mirella Luciani, Antonella Ciarelli, Grazia Bortone, Chiara Di Pancrazio, Diamante Rodomonti, Liana Teodori, Manuela Tittarelli
• Journal: Journal of immunoassay & immunochemistry (J Immunoassay Immunochem) Vol. 36 Issue 3 Pg. 253-64 ( 2015) ISSN: 1532-4230 [Electronic] England
• PMID: 24905982 (Publication Type: Journal Article)

Chemical References

• Antibodies, Monoclonal, Murine-Derived
• Immunoglobulin G

Topics

• African Horse Sickness (blood, diagnosis, immunology)
• Animals
• Antibodies, Monoclonal, Murine-Derived (chemistry, immunology)
• Dourine (blood, diagnosis, immunology)
• Fluorescent Antibody Technique, Indirect (methods)
• Horses
• Immunoglobulin G (blood, immunology)
• Mice