Abstract | AIMS: METHODS AND RESULTS: Quantitative killing tests against Escherichia coli and Staphylococcus aureus were performed both in the absence and in the presence of peptone with pairs of isosteric active chlorine and bromine compounds: hypochlorous and hypobromous acid (HOCl and HOBr), dichloro- and dibromoisocyanuric acid, chlorantine and bromantine (1,3-dibromo- and 1,3 dichloro-5,5-dimethylhydantoine), chloramine T and bromamine T (N-chloro- and N-bromo-4-methylbenzenesulphonamide sodium), and N-chloro- and N-bromotaurine sodium. To classify the bactericidal activities on a quantitative basis, an empirical coefficient named specific bactericidal activity (SBA), founded on the parameters of killing curves, was defined: SBA= mean log reductions/(mean exposure times x concentration) [mmol 1(-1) min (-1)]. In the absence of peptone, tests with washed micro-organisms revealed a throughout higher BA of bromine compounds with only slight differences between single substances. This was in contrast to chlorine compounds, whose killing times differed by a factor of more than four decimal powers. As a consequence, also the isosteric pairs showed according differences. In the presence of peptone, however, bromine compounds showed an increased loss of BA, which partly caused a reversal of efficacy within isosteric pairs. CONCLUSIONS: In medical practice, weakly oxidizing active chlorine compounds like chloramines have the highest potential as topical anti-infectives in the presence of proteinaceous material (mucous membranes, open wounds). Active bromine compounds, on the other hand, have their chance at insensitive body regions with low organic matter, for example skin surfaces. SIGNIFICANCE AND IMPACT OF THE STUDY: The expected protein load is one of the most important parameters for selection of a suited active halogen compound.
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Authors | W Gottardi, S Klotz, M Nagl |
Journal | Journal of applied microbiology
(J Appl Microbiol)
Vol. 116
Issue 6
Pg. 1427-37
(Jun 2014)
ISSN: 1365-2672 [Electronic] England |
PMID | 24905215
(Publication Type: Journal Article)
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Copyright | © 2014 The Society for Applied Microbiology. |
Chemical References |
- Anti-Infective Agents, Local
- Bromates
- Bromine Compounds
- Chloramines
- Chlorine Compounds
- Peptones
- Tosyl Compounds
- Triazines
- chloramine-T
- dibromoisocyanuric acid
- Taurine
- N-bromotaurine
- Hypochlorous Acid
- hypobromous acid
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Topics |
- Anti-Infective Agents, Local
(pharmacology)
- Bromates
(pharmacology)
- Bromine Compounds
(pharmacology)
- Chloramines
(pharmacology)
- Chlorine Compounds
(pharmacology)
- Escherichia coli
(drug effects)
- Hypochlorous Acid
(pharmacology)
- Peptones
(chemistry)
- Staphylococcus aureus
(drug effects)
- Taurine
(analogs & derivatives, pharmacology)
- Tosyl Compounds
(pharmacology)
- Triazines
(pharmacology)
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