Inactivated vaccine with adjuvants consisting of pattern recognition receptor agonists confers protection against avian influenza viruses in chickens.

Abstract

Use of adjuvant containing pathogen pattern recognition receptor agonists is one of the effective strategies to enhance the efficacy of licensed vaccines. In this study, we investigated the efficacy of avian influenza vaccines containing an adjuvant (CVCVA5) which was composed of polyriboinosinic polyribocytidylic, resiquimod, imiquimod, muramyl dipeptide and levomisole. Avian influenza vaccines adjuvanted with CVCVA5 were found to induce significantly higher titers of hemagglutiniton inhibition antibodies (P≤0.01) than those of commercial vaccines at 2-, 3- and 4-week post vaccination in both specific pathogen free (SPF) chickens and field application. Furthermore, virus shedding was reduced in SPF chickens immunized with H9-CVCVA5 vaccine after H9 subtype heterologous virus challenge. The ratios of both CD3(+)CD4(+) and CD3(+)CD8(+) lymphocytes were slowly elevated in chickens immunized with H9-CVCVA5 vaccine. Lymphocytes adoptive transfer study indicates that CD8(+) T lymphocyte subpopulation might have contributed to improved protection against heterologous virus challenge. Results of this study suggest that the adjuvant CVCVA5 was capable of enhancing the potency of existing avian influenza vaccines by increasing humoral and cellular immune response.

• Authors: Yinghua Tang, Jihu Lu, Peipei Wu, Zhenxing Liu, Zhen Tian, Guofei Zha, Hui Chen, Qiaochu Wang, Qiaoxiu Wang, Fengxiang Hou, Sang-Moo Kang, Jibo Hou
• Journal: Veterinary microbiology (Vet Microbiol) Vol. 172 Issue 1-2 Pg. 120-8 (Aug 06 2014) ISSN: 1873-2542 [Electronic] Netherlands
• PMID: 24894132 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier B.V. All rights reserved.

Chemical References

• Adjuvants, Immunologic
• Antibodies, Viral
• Influenza Vaccines
• Receptors, Pattern Recognition
• Vaccines, Inactivated

Topics

• Adjuvants, Immunologic (chemistry, pharmacology)
• Adoptive Transfer
• Animals
• Antibodies, Viral (blood)
• CD8-Positive T-Lymphocytes (immunology, transplantation, virology)
• Chickens (virology)
• Cross Protection
• Immunity, Active
• Immunophenotyping
• Influenza A virus (immunology)
• Influenza Vaccines (administration & dosage, immunology)
• Influenza in Birds (immunology, prevention & control, virology)
• Receptors, Pattern Recognition (agonists, genetics, immunology)
• Vaccination (veterinary)
• Vaccines, Inactivated
• Virus Shedding (drug effects)