[Effect of glimepiride on the glucose uptake of rat mandibular osteoblasts in hyperglycemia].

Abstract

OBJECTIVE:

To explore the effect of glimepiride on the glucose uptake as well as glucose transporter (GLUT)-1 and GLUT-3 expression levels of rat mandibular osteoblasts in hyperglycemia.

METHODS:

Primary osteoblasts were isolated and cultured. Then, the cells were placed in an osteogenic medium containing two glucose concentrations (5.5 and 16.5 mmol X L(-1)), with or without glimepiride (10 micromol x L(-1)). Glucose uptake was determined by employing 18F-deoxyglucose (18F-FDG) in the cells, and GLUT-1 and GLUT-3 expression levels were evaluated by Western blot analysis.

RESULTS:

Glucose at 16.5 mmol x L(-1) significantly inhibited 18F-FDG uptake and downregulated GLUT-3 protein expression in osteoblasts. Hyperglycemia increased GLUT-1 protein expression. Glimepiride significantly increased glucose uptake and upregulated GLUT-1 and GLUT-3.

CONCLUSION:

Glimepiride enhance the glucose transporter in rat osteoblasts at two different glucose concentrations.

• Authors: Pan Ma, Baosheng Tan, Hongchen Liu, Junli Ma, Bin Gu
• Journal: Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology (Hua Xi Kou Qiang Yi Xue Za Zhi) Vol. 32 Issue 2 Pg. 125-9 (Apr 2014) ISSN: 1000-1182 [Print] China
• PMID: 24881204 (Publication Type: Journal Article)

Chemical References

• Glucose Transporter Type 1
• SLC2A1 protein, human
• Sulfonylurea Compounds
• Fluorodeoxyglucose F18
• glimepiride
• Glucose

Topics

• Animals
• Fluorodeoxyglucose F18
• Glucose
• Glucose Transporter Type 1
• Hyperglycemia
• Mandible
• Osteoblasts
• Rats
• Sulfonylurea Compounds