[Effect of glimepiride on the glucose uptake of rat mandibular osteoblasts in hyperglycemia].

To explore the effect of glimepiride on the glucose uptake as well as glucose transporter (GLUT)-1 and GLUT-3 expression levels of rat mandibular osteoblasts in hyperglycemia.
Primary osteoblasts were isolated and cultured. Then, the cells were placed in an osteogenic medium containing two glucose concentrations (5.5 and 16.5 mmol X L(-1)), with or without glimepiride (10 micromol x L(-1)). Glucose uptake was determined by employing 18F-deoxyglucose (18F-FDG) in the cells, and GLUT-1 and GLUT-3 expression levels were evaluated by Western blot analysis.
Glucose at 16.5 mmol x L(-1) significantly inhibited 18F-FDG uptake and downregulated GLUT-3 protein expression in osteoblasts. Hyperglycemia increased GLUT-1 protein expression. Glimepiride significantly increased glucose uptake and upregulated GLUT-1 and GLUT-3.
Glimepiride enhance the glucose transporter in rat osteoblasts at two different glucose concentrations.
AuthorsPan Ma, Baosheng Tan, Hongchen Liu, Junli Ma, Bin Gu
JournalHua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology (Hua Xi Kou Qiang Yi Xue Za Zhi) Vol. 32 Issue 2 Pg. 125-9 (Apr 2014) ISSN: 1000-1182 [Print] China
PMID24881204 (Publication Type: English Abstract, Journal Article)
Chemical References
  • Glucose Transporter Type 1
  • SLC2A1 protein, human
  • Sulfonylurea Compounds
  • Fluorodeoxyglucose F18
  • glimepiride
  • Glucose
  • Animals
  • Fluorodeoxyglucose F18
  • Glucose
  • Glucose Transporter Type 1
  • Hyperglycemia
  • Mandible
  • Osteoblasts
  • Rats
  • Sulfonylurea Compounds

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