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Prophylaxis and therapy of pandemic H1N1 virus infection using egg yolk antibody.

Abstract
Influenza A virus infects the human respiratory system and causes acute and fatal pulmonary diseases. The emergence of drug-resistant viral strains highlights the need for alternative therapeutic approaches. In this work, IgY antibody was raised in immunized laying hens, and its antiviral activity was evaluated in the context of passive immunization. With inactivated whole H1N1 virus, high-titer IgY antibody 9.18 mg/mL egg yolk was induced by the eighth week after immunization. Western blotting and the hemagglutination inhibition (HI) test demonstrated that the IgY antibody could specifically bind the neuraminidase and hemagglutinin of the H1N1 virus. In the plaque reduction assay, the IgY antibody reduced the H1N1 viral infection in MDCK (Madin-Darby canine kidney) cells. In a mouse model, the anti-H1N1 IgY antibody exhibited in vivo protection by reducing the infectious titer of the virus in the lung while maintaining the weight and normal structure of the lung tissue. Additionally, the anti-H1N1 IgY antibody exhibited protective activity comparable to the neuraminidase inhibitor oseltamivir. These results demonstrated that IgY can be easily produced and can offers an effective alternative approach for influenza control.
AuthorsYuan-e Yang, Junlin Wen, Suqing Zhao, Kun Zhang, Yingliang Zhou
JournalJournal of virological methods (J Virol Methods) Vol. 206 Pg. 19-26 (Sep 2014) ISSN: 1879-0984 [Electronic] Netherlands
PMID24880066 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2014 Elsevier B.V. All rights reserved.
Chemical References
  • Antibodies, Viral
  • IgY
  • Immunoglobulins
Topics
  • Animals
  • Antibodies, Viral (administration & dosage, immunology)
  • Body Weight
  • Disease Models, Animal
  • Egg Yolk (immunology)
  • Female
  • Humans
  • Immunization, Passive (methods)
  • Immunoglobulins (administration & dosage, immunology)
  • Influenza A Virus, H1N1 Subtype (immunology)
  • Lung (pathology, virology)
  • Mice, Inbred BALB C
  • Neutralization Tests
  • Orthomyxoviridae Infections (prevention & control, therapy)
  • Treatment Outcome
  • Viral Load
  • Viral Plaque Assay

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