A novel VHH antibody targeting the B cell-activating factor for B-cell lymphoma.

Abstract

OBJECTIVE:

To construct an immune alpaca phage display library, in order to obtain a single domain anti-BAFF (B cell-activating factor) antibody.

METHODS:

Using phage display technology, we constructed an immune alpaca phage display library, selected anti-BAFF single domain antibodies (sdAbs), cloned three anti-BAFF single-domain antibody genes into expression vector pSJF2, and expressed them efficiently in Escherichia coli. The affinity of different anti-BAFF sdAbs were measured by Bio layer interferometry. The in vitro biological function of three sdAbs was investigated by cell counting kit-8 (CCK-8) assay and a competitive enzyme-linked immunosorbent assay (ELISA).

RESULTS:

We obtained three anti-BAFF single domain antibodies (anti-BAFF64, anti-BAFF52 and anti-BAFFG3), which were produced in high yield in Escherichia coli and inhibited tumor cell proliferation in vitro.

CONCLUSION:

The selected anti-BAFF antibodies could be candidates for B-cell lymphoma therapies.

• Authors: Wen Wu, Shenghua Li, Weijing Zhang, Jian Sun, Guangda Ren, Quanchao Dong
• Journal: International journal of molecular sciences (Int J Mol Sci) Vol. 15 Issue 6 Pg. 9481-96 (May 28 2014) ISSN: 1422-0067 [Electronic] Switzerland
• PMID: 24879522 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• B-Cell Activating Factor
• Immunoglobulin Heavy Chains
• Peptide Library
• Single-Domain Antibodies

Topics

• Amino Acid Sequence
• Animals
• B-Cell Activating Factor (immunology)
• B-Lymphocytes (drug effects, immunology, pathology)
• Camelids, New World
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Cloning, Molecular
• Escherichia coli (genetics)
• Humans
• Immunoglobulin Heavy Chains (chemistry, genetics, immunology, pharmacology)
• Lymphoma, B-Cell (drug therapy, immunology)
• Molecular Sequence Data
• Peptide Library
• Sequence Alignment
• Single-Domain Antibodies (chemistry, genetics, immunology, pharmacology)