Abstract | OBJECTIVE: To construct an immune alpaca phage display library, in order to obtain a single domain anti-BAFF ( B cell-activating factor) antibody. METHODS: Using phage display technology, we constructed an immune alpaca phage display library, selected anti-BAFF single domain antibodies (sdAbs), cloned three anti-BAFF single-domain antibody genes into expression vector pSJF2, and expressed them efficiently in Escherichia coli. The affinity of different anti-BAFF sdAbs were measured by Bio layer interferometry. The in vitro biological function of three sdAbs was investigated by cell counting kit-8 (CCK-8) assay and a competitive enzyme-linked immunosorbent assay (ELISA). RESULTS: We obtained three anti-BAFF single domain antibodies (anti-BAFF64, anti-BAFF52 and anti-BAFFG3), which were produced in high yield in Escherichia coli and inhibited tumor cell proliferation in vitro. CONCLUSION:
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Authors | Wen Wu, Shenghua Li, Weijing Zhang, Jian Sun, Guangda Ren, Quanchao Dong |
Journal | International journal of molecular sciences
(Int J Mol Sci)
Vol. 15
Issue 6
Pg. 9481-96
(May 28 2014)
ISSN: 1422-0067 [Electronic] Switzerland |
PMID | 24879522
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- B-Cell Activating Factor
- Immunoglobulin Heavy Chains
- Peptide Library
- Single-Domain Antibodies
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Topics |
- Amino Acid Sequence
- Animals
- B-Cell Activating Factor
(immunology)
- B-Lymphocytes
(drug effects, immunology, pathology)
- Camelids, New World
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- Cloning, Molecular
- Escherichia coli
(genetics)
- Humans
- Immunoglobulin Heavy Chains
(chemistry, genetics, immunology, pharmacology)
- Lymphoma, B-Cell
(drug therapy, immunology)
- Molecular Sequence Data
- Peptide Library
- Sequence Alignment
- Single-Domain Antibodies
(chemistry, genetics, immunology, pharmacology)
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