The
membrane protein tyrosine phosphatase receptor U (PTPRU) has been shown to function as a negative regulator of adhesion and proliferation in certain
cancer cell types, primarily through its dephosphorylation of β-
catenin and inhibition of subsequent downstream signaling. In the present study, we set out to characterize the role of PTPRU in
glioma and found that, while the expression of full-length PTPRU
protein is low in these
tumors, a number of non-full-length PTPRU
isoforms are highly expressed. Among these
isoforms, one in particular is localized to the nucleus, and its expression is increased in
glioma tissues in a manner that positively correlates with
malignancy grade.
Short hairpin RNA knockdown of endogenous PTPRU in human and rat
glioma cell lines suppressed proliferation, survival, invasion, migration, adhesion and vasculogenic tube formation in vitro, as well as intracranial
tumor progression in vivo. In addition, knocking down PTPRU reduced
tyrosine phosphorylation (pY) and transcriptional activity of β-
catenin, and we were able to specifically rescue the cell migration defect by expressing a LEF1-β-catenin fusion
protein in PTPRU-depleted cells. PTPRU knockdown also led to increased
tyrosine pY of the
E3 ubiquitin ligase c-Cbl and to the destabilization of several focal adhesion
proteins. Taken together, our findings demonstrate that endogenous PTPRU promote
glioma progression through their effect on β-
catenin and focal adhesion signaling.