Liposomal inhibition of acrolein-induced injury in rat cultured urothelial cells.

Abstract

PURPOSE:

To study the protection offered by empty liposomes (LPs) alone against acrolein-induced changes in urothelial cell viability and explored uptake of LPs by primary (rat) urothelial cells.

METHODS:

Acrolein was used as a means to induce cellular damage and reduce urothelial cellular viability. The effect of acrolein or liposomal treatment on cellular proliferation was studied using 5-bromo-2'-deoxy-uridine assay. Cytokine release was measured after urothelial cells were exposed to acrolein. Temperature-dependent uptake study was carried out for fluorescent-labeled LPs using confocal microscopy.

RESULTS:

Liposome pretreatment protected against acrolein-induced decrease in urothelial cell proliferation. LPs also significantly affected the acrolein-induced cytokine (interferon-gamma) release offering protection to the urothelial cells against acrolein damage. We also observed a temperature-dependent urothelial uptake of fluorescent-labeled LPs occurred at 37 °C (but not at 4 °C).

CONCLUSIONS:

Empty LPs alone provide a therapeutic efficacy against acrolein-induced changes in urothelial cell viability and may be a promising local therapy for bladder diseases. Hence, our preliminary evidence provides support for liposome-therapy for urothelial protection and possible repair.

• Authors: J Nirmal, A S Wolf-Johnston, M B Chancellor, P Tyagi, M Anthony, J Kaufman, L A Birder
• Journal: International urology and nephrology (Int Urol Nephrol) Vol. 46 Issue 10 Pg. 1947-52 (Oct 2014) ISSN: 1573-2584 [Electronic] Netherlands
• PMID: 24875005 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Liposomes
• Acrolein
• Interferon-gamma
• Adenosine Triphosphate

Topics

• Acrolein (toxicity)
• Adenosine Triphosphate (metabolism)
• Animals
• Cell Line
• Cell Proliferation (drug effects)
• Cells, Cultured
• Dose-Response Relationship, Drug
• Female
• Interferon-gamma (metabolism)
• Liposomes (pharmacology)
• Microscopy, Confocal
• Rats
• Rats, Sprague-Dawley
• Urothelium (cytology, drug effects, metabolism)