Abstract | PURPOSE: To study the protection offered by empty liposomes (LPs) alone against acrolein-induced changes in urothelial cell viability and explored uptake of LPs by primary (rat) urothelial cells. METHODS:
Acrolein was used as a means to induce cellular damage and reduce urothelial cellular viability. The effect of acrolein or liposomal treatment on cellular proliferation was studied using 5-bromo-2'-deoxy-uridine assay. Cytokine release was measured after urothelial cells were exposed to acrolein. Temperature-dependent uptake study was carried out for fluorescent-labeled LPs using confocal microscopy. RESULTS:
Liposome pretreatment protected against acrolein-induced decrease in urothelial cell proliferation. LPs also significantly affected the acrolein-induced cytokine ( interferon-gamma) release offering protection to the urothelial cells against acrolein damage. We also observed a temperature-dependent urothelial uptake of fluorescent-labeled LPs occurred at 37 °C (but not at 4 °C). CONCLUSIONS: Empty LPs alone provide a therapeutic efficacy against acrolein-induced changes in urothelial cell viability and may be a promising local therapy for bladder diseases. Hence, our preliminary evidence provides support for liposome- therapy for urothelial protection and possible repair.
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Authors | J Nirmal, A S Wolf-Johnston, M B Chancellor, P Tyagi, M Anthony, J Kaufman, L A Birder |
Journal | International urology and nephrology
(Int Urol Nephrol)
Vol. 46
Issue 10
Pg. 1947-52
(Oct 2014)
ISSN: 1573-2584 [Electronic] Netherlands |
PMID | 24875005
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
- Liposomes
- Acrolein
- Interferon-gamma
- Adenosine Triphosphate
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Topics |
- Acrolein
(toxicity)
- Adenosine Triphosphate
(metabolism)
- Animals
- Cell Line
- Cell Proliferation
(drug effects)
- Cells, Cultured
- Dose-Response Relationship, Drug
- Female
- Interferon-gamma
(metabolism)
- Liposomes
(pharmacology)
- Microscopy, Confocal
- Rats
- Rats, Sprague-Dawley
- Urothelium
(cytology, drug effects, metabolism)
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