Abstract | INTRODUCTION: METHODS: HEK293 cells expressing specific individual recombinant subunits of α(1A), α(1B), α(1C), and α(1E); β(3); and α(2)δ of human neuronal VGCCs were exposed to antibodies from 3 LEMS patients, 1 patient with small-cell lung carcinoma, and 1 with myasthenia gravis. RESULTS: CONCLUSIONS:
Autoantibodies from LEMS patients bind directly to multiple VGCC α(1) subunits as well as the β(3) subunit. Thus, multiple components of the presynaptic VGCC complex are prospective targets for antibodies in LEMS.
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Authors | Ravindra K Hajela, Kristin M Huntoon, William D Atchison |
Journal | Muscle & nerve
(Muscle Nerve)
Vol. 51
Issue 2
Pg. 176-84
(Feb 2015)
ISSN: 1097-4598 [Electronic] United States |
PMID | 24862203
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Copyright | © 2014 Wiley Periodicals, Inc. |
Chemical References |
- Autoantibodies
- Calcium Channels
- Protein Subunits
- Green Fluorescent Proteins
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Topics |
- Autoantibodies
(immunology)
- Calcium Channels
(genetics, immunology, metabolism)
- Carcinoma, Small Cell
(blood, immunology)
- Green Fluorescent Proteins
(genetics, metabolism)
- HEK293 Cells
- Humans
- Lambert-Eaton Myasthenic Syndrome
(blood, immunology)
- Protein Subunits
(genetics, metabolism)
- Transfection
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