Beneficial influence of physical exercise following status epilepticus in the immature brain of rats.

Abstract

Studies in adult animals have demonstrated a beneficial effect of physical exercise on epileptic insults. Although the effects of physical exercise on the mature nervous system are well documented, its influence on the developing nervous system subjected to injuries in childhood has been little explored. The purpose of our study was to investigate whether a physical exercise program applied during brain development could influence the hippocampal plasticity of rats submitted to status epilepticus (SE) induced by pilocarpine model at two different ages of the postnatal period. Male Wistar rats aged 18 (P18) and 28 (P28) days were randomly divided into four groups: Control (CTRL), Exercise (EX), SE (SE) and SE Exercise (SE/EX) (n=17 per group). After the aerobic exercise program, histological and behavioral (water maze) analyses were performed. Our results showed that only animals subjected to pilocarpine-induced SE at P28 presented spontaneous seizures during the observational period. A significant reduction in seizure frequency was observed in the SE/EX group compared to the SE group. In adulthood, animals submitted to early-life SE displayed impairment in long-term memory in the water maze task, while the exercise program reversed this deficit. Reduced mossy fiber sprouting in the dentate gyrus was noted in animals that presented spontaneous seizures (SE/EX vs SE). Exercise increased cell proliferation (Ki-67 staining) and anti-apoptotic response (bcl-2 staining) and reduced pro-apoptotic response (Bax staining) in animals of both ages of SE induction (P18/28). Exercise also modified the brain-derived neurotrophic factor (BDNF) levels in EX and SE/EX animals. Our findings indicate that in animals subjected to SE in the postnatal period a physical exercise program brings about beneficial effects on seizure frequency and hippocampal plasticity in later stages of life.

• Authors: F G Novaes Gomes, S Gomes Da Silva, E A Cavalheiro, R M Arida
• Journal: Neuroscience (Neuroscience) Vol. 274 Pg. 69-81 (Aug 22 2014) ISSN: 1873-7544 [Electronic] United States
• PMID: 24857853 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 IBRO. Published by Elsevier Ltd. All rights reserved.

Chemical References

• Brain-Derived Neurotrophic Factor
• Pilocarpine

Topics

• Age Factors
• Animals
• Apoptosis
• Brain (metabolism, physiopathology)
• Brain-Derived Neurotrophic Factor (metabolism)
• Cell Proliferation
• Exercise Therapy
• Male
• Maze Learning (physiology)
• Neuronal Plasticity
• Pilocarpine
• Rats
• Rats, Wistar
• Status Epilepticus (physiopathology, therapy)