RNA interference (RNAi) mediated stable knockdown of protein casein kinase 2-alpha (CK2α) inhibits migration and invasion and enhances cisplatin-induced apoptosis in HEp-2 laryngeal carcinoma cells.

Abstract

Laryngeal carcinoma is a common malignant neoplasm occurring in the head and neck, threatening human health. Protein casein kinase 2-alpha (CK2α) has been indicated to participate in the pathogenesis of this cancer; however, the underlying mechanisms still need to be elucidated. In this study, short hairpin RNA (shRNA)-mediated RNA interference (RNAi) technology was utilized to inhibit the CK2α expression in HEp-2 laryngeal carcinoma cells. Results showed that both mRNA and protein expression levels of endogenous CK2α were markedly decreased in HEp-2 cells transfected with CK2α specific shRNA. Transwell assays revealed that stable knockdown of CK2α significantly inhibited the migration and invasion of HEp-2 cells. As compared with cells treated with negative control shRNA, epithelial cadherin (E-cadherin) expression was increased, but snail, slug and vimentin were decreased in cells transfected with CK2α shRNA, indicating that inhibition of CK2α expression may suppress the epithelial-mesenchymal transition (EMT) process of laryngeal carcinoma in vitro. Moreover, suppression of CK2α was found to enhance the apoptosis induced by cisplatin in laryngeal carcinoma cells, probably through inhibition of permeability glycoprotein (P-glycoprotein) and multidrug-resistance protein (MRP1). In conclusion, our study may provide a promising therapeutic strategy for human laryngeal carcinoma by targeting CK2α.

• Authors: Fang Zhang, Bo Yang, Shengli Shi, Xuejun Jiang
• Journal: Acta histochemica (Acta Histochem) Vol. 116 Issue 6 Pg. 1000-6 (Jul 2014) ISSN: 1618-0372 [Electronic] Germany
• PMID: 24831064 (Publication Type: Journal Article)
• Copyright: Copyright © 2014 Elsevier GmbH. All rights reserved.

Chemical References

• Antigens, CD
• Antineoplastic Agents
• CDH1 protein, human
• Cadherins
• RNA, Small Interfering
• Vimentin
• Casein Kinase II
• Cisplatin

Topics

• Antigens, CD
• Antineoplastic Agents (pharmacology)
• Apoptosis (drug effects)
• Cadherins (genetics, metabolism)
• Casein Kinase II (genetics, metabolism)
• Cell Line, Tumor
• Cell Movement
• Cisplatin (pharmacology)
• Drug Screening Assays, Antitumor
• Gene Expression
• Gene Knockdown Techniques
• Humans
• Laryngeal Neoplasms (drug therapy, enzymology, pathology)
• Neoplasm Invasiveness
• RNA Interference
• RNA, Small Interfering (genetics)
• Vimentin (genetics, metabolism)