Assessment of clonality in T-cell large granular lymphocytic leukemia: flow cytometric T cell receptor Vβ repertoire and T cell receptor gene rearrangement.
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| Abstract |
The usefulness of flow cytometric variable β-chain repertoire (FC-Vβ) and T-cell receptor gene rearrangement (TCR-GR) analyses for differentiating T-cell large granular lymphocytic leukemia (T-LGLL) from reactive T-large granular lymphocyte (T-LGL) lymphocytosis has been insufficiently studied to date. In this study, we analyzed the diagnostic value of TCR-GR and FC-Vβ analysis in T-LGLL, and compared these results. In our study, FC-Vβ analysis was positive in all cases of T-LGLL, and clonality assessment of FC-Vβ had equal sensitivity and specificity to GeneScanning analysis but was more sensitive than heteroduplex analysis. Suspected T-cell clonality can best be addressed by evaluating two TCR targets (TCRβ and TCRγ), either in parallel or consecutively. Signal transducer and activator of transcription 3 (STAT3) mutation may provide a diagnostic tool for classifying some cases of T-LGL lymphocytosis as true T-LGLL. Our results further demonstrate a significant correlation of STAT3 mutation with pure red cell aplasia, neutropenia, hepatomegaly, β2-microglobulin and anemia.
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| Authors | Zhi-Yuan Qiu, Wen-Yi Shen, Lei Fan, Li Wang, Hui Yu, Chun Qiao, Yu-Jie Wu, Rui-Nan Lu, Jun Qian, Guang-Sheng He, Wei Xu, Jian-Yong Li |
| Journal | Leukemia & lymphoma (Leuk Lymphoma) Vol. 56 Issue 2 Pg. 324-31 (Feb 2015) ISSN: 1029-2403 [Electronic] United States |
| PMID | 24828862 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
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