Abstract | BACKGROUND:
Urotensin II (UII), a potent vasoconstrictive peptide, is able to stimulate phenotypic differentiation of adventitial fibroblasts. This study aimed to determine the effect of UII on monocyte chemoattractant protein-1 (MCP-1) expression in rat aortic adventitial fibroblasts, so as to explore possible mechanisms in the development of vascular inflammation. METHODS: Growth-arrested adventitial fibroblasts were incubated in serum-free medium with UII (10(-10)-10(-7) mol/L) and inhibitors of signal transduction pathways for 1 to 24 hours. MCP-1 mRNA and protein expression and secretion were determined by RT-PCR, Western blotting analysis and enzyme-linked immunosorbent assay (ELISA), respectively. RESULTS: CONCLUSION:
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Authors | Yonggang Zhang, Shilin Bao, Zejian Kuang, Yanjun Ma, Yanchao Hu, Yanyan Mao |
Journal | Chinese medical journal
(Chin Med J (Engl))
Vol. 127
Issue 10
Pg. 1907-12
( 2014)
ISSN: 2542-5641 [Electronic] China |
PMID | 24824254
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Chemokine CCL2
- RNA, Messenger
- Urotensins
- urotensin II
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Topics |
- Adventitia
(cytology)
- Animals
- Aorta
(cytology)
- Cells, Cultured
- Chemokine CCL2
(genetics, metabolism)
- Fibroblasts
(drug effects, metabolism)
- Male
- RNA, Messenger
(genetics)
- Rats
- Rats, Sprague-Dawley
- Reverse Transcriptase Polymerase Chain Reaction
- Urotensins
(pharmacology)
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