Abstract | BACKGROUND: METHODS: We tested the hypotheses that progesterone receptors (PR) are expressed in ARH OFQ/N neurons by immunohistochemistry and ORL-1 is expressed in POMC neurons that project to the MPN by combining Fluoro-Gold injection into the MPN and double-label fluorescent in situ hybridization (FISH). We also hypothesized that estradiol increases coexpression of PR-OFQ/N and ORL-1-POMC in ARH neurons of ovariectomized rats. RESULTS: The number of PR- and OFQ/N-immunopositive ARH neurons was increased as was their colocalization by estradiol treatment. FISH for ORL-1 and POMC mRNA revealed a subpopulation of ARH neurons that was triple labeled, indicating these neurons project to the MPN and coexpress ORL-1 and POMC mRNA. Estradiol was shown to upregulate ORL-1 and POMC expression in MPN-projecting ARH neurons. CONCLUSION:
Estradiol upregulates the ARH OFQ/N-ORL-1 system projecting to the MPN that regulates lordosis.
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Authors | Nayna M Sanathara, Justine Moreas, Matthew Mahavongtrakul, Kevin Sinchak |
Journal | Neuroendocrinology
(Neuroendocrinology)
Vol. 100
Issue 2-3
Pg. 103-18
( 2014)
ISSN: 1423-0194 [Electronic] Switzerland |
PMID | 24821192
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
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Copyright | © 2014 S. Karger AG, Basel. |
Chemical References |
- Estrogens
- Opioid Peptides
- RNA, Messenger
- Receptors, Opioid
- Receptors, Progesterone
- Estradiol
- Pro-Opiomelanocortin
- nociceptin
- Nociceptin Receptor
- Oprl protein, rat
|
Topics |
- Animals
- Arcuate Nucleus of Hypothalamus
(physiology)
- Estradiol
(administration & dosage, metabolism)
- Estrogens
(administration & dosage, metabolism)
- Female
- Neural Pathways
(physiology)
- Neurons
(physiology)
- Opioid Peptides
(metabolism)
- Ovariectomy
- Posture
(physiology)
- Preoptic Area
(physiology)
- Pro-Opiomelanocortin
(metabolism)
- RNA, Messenger
(metabolism)
- Rats, Long-Evans
- Receptors, Opioid
(metabolism)
- Receptors, Progesterone
(metabolism)
- Sexual Behavior, Animal
(physiology)
- Nociceptin Receptor
|