Heterogeneity of microRNAs expression in cervical cancer cells: over-expression of miR-196a.

Abstract

In recent years, the study of microRNAs associated with neoplastic processes has increased. Patterns of microRNA expression in different cell lines and different kinds of tumors have been identified; however, little is known about the alterations in regulatory pathways and genes involved in aberrant set of microRNAs. The identification of these altered microRNAs in several cervical cancer cells and potentially deregulated pathways involved constitute the principal goals of the present study. In the present work, the expression profiles of cellular microRNAs in Cervical Cancer tissues and cell lines were explored using microRNA microarray, Affymetrix. The most over-expressed was miR-196a, which was evaluated by real time PCR, and HOXC8 protein as potential target by immunohistochemistry assay. One hundred and twenty three human microRNAs differentially expressed in the cell tumor, 64 (52%) over-expressed and 59 (48%) under-expressed were observed. Among the microRNAs over-expressed, we focused on miR-196a; at present this microRNA is poorly studied in CC. The expression of this microRNA was evaluated by qRT-PCR, and HOXC8 by immunohistochemistry assay. There is not a specific microRNA expression profile in the CC cells, neither a microRNA related to HPV presence. Furthermore, the miR-196a was over-expressed, while an absence of HOXC8 expression was observed. We suggest that miR-196a could be played as oncomiR in CC.

• Authors: Vanessa Villegas-Ruiz, Sergio Juárez-Méndez, Oscar A Pérez-González, Hugo Arreola, Lucero Paniagua-García, Miriam Parra-Melquiadez, Raúl Peralta-Rodríguez, Ricardo López-Romero, Alberto Monroy-García, Alejandra Mantilla-Morales, Guillermo Gómez-Gutiérrez, Edgar Román-Bassaure, Mauricio Salcedo
• Journal: International journal of clinical and experimental pathology (Int J Clin Exp Pathol) Vol. 7 Issue 4 Pg. 1389-401 ( 2014) ISSN: 1936-2625 [Electronic] United States
• PMID: 24817935 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• HOXC8 protein, human
• Homeodomain Proteins
• MIRN196 microRNA, human
• MicroRNAs

Topics

• Cell Line
• Cell Line, Tumor
• Cervix Uteri (metabolism, pathology)
• Female
• Gene Expression Regulation, Neoplastic (genetics, physiology)
• Homeodomain Proteins (genetics, metabolism)
• Humans
• Immunohistochemistry
• MicroRNAs (genetics, metabolism)
• Microarray Analysis
• Up-Regulation (genetics, physiology)
• Uterine Cervical Neoplasms (genetics, metabolism, physiopathology)