Spectroscopic studies on the antioxidant activity of ellagic acid.

Abstract

Ellagic acid (EA, C14H6O8) is a natural dietary polyphenol whose benefits in a variety of diseases shown in epidemiological and experimental studies involve anti-inflammation, anti-proliferation, anti-angiogenesis, anticarcinogenesis and anti-oxidation properties. In vitro radical scavenging and antioxidant capacity of EA were clarified using different analytical methodologies such as total antioxidant activity determination by ferric thiocyanate, hydrogen peroxide scavenging, 1,1-diphenyl-2-picryl-hydrazyl free radical (DPPH) scavenging, 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging activity and superoxide anion radical scavenging, ferrous ions (Fe2+) chelating activity and ferric ions (Fe3+) reducing ability. EA inhibited 71.2% lipid peroxidation of a linoleic acid emulsion at 45 μg/mL concentration. On the other hand, butylated hydroxytoluene (BHT), butylated hydroxyanisole (BHA), α-tocopherol and ascorbic acid displayed 69.8%, 66.8%, 64.5% and 59.7% inhibition on the peroxidation of linoleic acid emulsion at the same concentration, respectively. In addition, EA had an effective DPPH• scavenging, ABTS+ scavenging, superoxide anion radical scavenging, hydrogen peroxide scavenging, ferric ions (Fe3+) reducing power and ferrous ions (Fe2+) chelating activities. Also, those various antioxidant activities were compared to BHA, BHT, α-tocopherol and ascorbic acid as references antioxidant compounds. These results suggested that EA can be used in the pharmacological, food industry and medicine because of these properties.

• Authors: Ismail Kilic, Yeşim Yeşiloğlu, Yüksel Bayrak
• Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (Spectrochim Acta A Mol Biomol Spectrosc) Vol. 130 Pg. 447-52 (Sep 15 2014) ISSN: 1873-3557 [Electronic] England
• PMID: 24813273 (Publication Type: Journal Article)
• Copyright: Copyright © 2014 Elsevier B.V. All rights reserved.

Chemical References

• Antioxidants
• Biphenyl Compounds
• Chelating Agents
• Emulsions
• Free Radical Scavengers
• Free Radicals
• Lipids
• Picrates
• Thiocyanates
• Superoxides
• Ellagic Acid
• Butylated Hydroxytoluene
• Butylated Hydroxyanisole
• ferric thiocyanate
• Linoleic Acid
• Hydrogen Peroxide
• 1,1-diphenyl-2-picrylhydrazyl
• Iron
• alpha-Tocopherol
• Ascorbic Acid
• Oxygen

Topics

• Antioxidants (chemistry)
• Ascorbic Acid (chemistry)
• Biphenyl Compounds (chemistry)
• Butylated Hydroxyanisole (chemistry)
• Butylated Hydroxytoluene (chemistry)
• Chelating Agents (chemistry)
• Ellagic Acid (chemistry)
• Emulsions
• Free Radical Scavengers (chemistry)
• Free Radicals
• Hydrogen Peroxide (chemistry)
• Iron (chemistry)
• Linoleic Acid (chemistry)
• Lipids (chemistry)
• Oxygen (chemistry)
• Picrates (chemistry)
• Spectrophotometry
• Superoxides (chemistry)
• Thiocyanates (chemistry)
• Time Factors
• alpha-Tocopherol (chemistry)