Abstract | PURPOSE: Downregulation of miRNA expression has been identified as a novel feature of renal cell carcinoma (RCC). Recently, miR-129-2 is well known to be frequently reduced by DNA methylation and has anti- tumor effects in various tumors but so far not in RCC. The aim of this study was to investigate the clinical significance and the role of it in RCC. METHODS: The expression levels of miR-129-3p and miR-129-5p, two mature products of miR-129-2, were determined by real-time quantitative reverse transcription PCR in 69 cases of paired different kidney tumors and normal tissues and clear cell RCC (ccRCC) cell lines. The roles of them in RCC cells were assessed by functional analyses. Protein expression was detected by Western blot. RESULTS: miR-129-3p, but not miR-129-5p, was widely attenuated in human ccRCC, and chromophobe RCC. miR-129-3p could yield 73.5 % accuracy in discriminating ccRCCs from normal tissues. The relative miR-129-3p expression significantly differed between malignant and benign kidney tumors. Importantly, low miR-129-3p levels were associated with short disease-free and overall survival. Ectopic expression of miR-129-3p robustly impaired RCC cell migratory and invasive properties, but had no impact on cell viability and cell cycle distribution. Finally, miR-129-3p decreased multiple metastasis-related genes in RCC cells, including SOX4, phosphorylation of focal adhesion kinase and MMP-2/9 expression. CONCLUSIONS: miR-129-3p may act as a promising diagnostic biomarker for discriminating ccRCC from benign tumors and normal tissues and an independent prognostic biomarker in ccRCC. miR-129-3p may exert its anti-metastatic function through modulating multiple targets.
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Authors | Xuanyu Chen, Anming Ruan, Xuegang Wang, Weiwei Han, Rong Wang, Ning Lou, Hailong Ruan, Bin Qiu, Hongmei Yang, Xiaoping Zhang |
Journal | Journal of cancer research and clinical oncology
(J Cancer Res Clin Oncol)
Vol. 140
Issue 8
Pg. 1295-304
(Aug 2014)
ISSN: 1432-1335 [Electronic] Germany |
PMID | 24802708
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers, Tumor
- MicroRNAs
- Mirn129 microRNA, human
- MMP2 protein, human
- Matrix Metalloproteinase 2
- MMP9 protein, human
- Matrix Metalloproteinase 9
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Topics |
- Adult
- Aged
- Biomarkers, Tumor
(genetics, metabolism)
- Carcinoma, Renal Cell
(genetics, metabolism, mortality, secondary)
- Cell Line, Tumor
- Cell Movement
- Cell Survival
- Down-Regulation
- Female
- Gene Expression
- Gene Expression Regulation, Neoplastic
- HEK293 Cells
- Humans
- Kaplan-Meier Estimate
- Kidney Neoplasms
(genetics, metabolism, mortality, pathology)
- Male
- Matrix Metalloproteinase 2
(genetics, metabolism)
- Matrix Metalloproteinase 9
(genetics, metabolism)
- MicroRNAs
(genetics, metabolism)
- Middle Aged
- Neoplasm Invasiveness
- Prognosis
- RNA Interference
- ROC Curve
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