HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

CDR3 analysis of TCR Vβ repertoire of CD8⁺ T cells from chickens infected with Eimeria maxima.

Abstract
CD8(+) T cells play a major role in the immune protection of host against the reinfection of Eimeria maxima, the most immunogenic species of eimerian parasites in chickens. To explore the dominant complementarity-determining regions 3 (CDR3) of CD8(+) T cell populations induced by the infection of this parasite, sequence analysis was performed in this study for CDR3 of CD8(+) T cells from E. maxima infected chickens. After 5 days post the third or forth infection, intraepithelial lymphocytes were isolated from the jejunum of bird. CD3(+)CD8(+) T cells were sorted and subjected to total RNA isolation and cDNA preparation. PCR amplification and cloning of the loci between Vβ1 and Cβ was conducted for the subsequent sequencing of CDR3 of T cell receptor (TCR). After the forth infection, 2 birds exhibited two same frequent TCR CDR3 sequences, i.e., AKQDWGTGGYSNMI and AGRVLNIQY; while the third bird showed two different frequent TCR CDR3 sequences, AKQGARGHTPLN and AKQDIEVRGPNTPLN. No frequent CDR3 sequence was detected from uninfected birds, though AGRVLNIQY was also found in two uninfected birds. Our result preliminarily demonstrates that frequent CDR3 sequences may exist in E. maxima immunized chickens, encouraging the mining of the immunodominant CD8(+) T cells against E. maxima infection.
AuthorsChao Ren, Guangwen Yin, Mei Qin, Jingxia Suo, Qiyao Lv, Li Xie, Yunzhou Wang, Xiaoxi Huang, Yuchen Chen, Xianyong Liu, Xun Suo
JournalExperimental parasitology (Exp Parasitol) Vol. 143 Pg. 1-4 (Aug 2014) ISSN: 1090-2449 [Electronic] United States
PMID24801021 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2014 Elsevier Inc. All rights reserved.
Chemical References
  • Complementarity Determining Regions
  • DNA, Complementary
  • Immunodominant Epitopes
  • RNA, Protozoan
  • Receptors, Antigen, T-Cell, alpha-beta
Topics
  • Amino Acid Sequence
  • Animals
  • CD8-Positive T-Lymphocytes (immunology)
  • Cell Separation (veterinary)
  • Chickens (immunology, parasitology)
  • Coccidiosis (immunology, parasitology, veterinary)
  • Complementarity Determining Regions (chemistry, genetics, immunology)
  • DNA, Complementary (genetics)
  • Eimeria (immunology)
  • Flow Cytometry (veterinary)
  • Immunodominant Epitopes (immunology)
  • Poultry Diseases (immunology, parasitology)
  • RNA, Protozoan (genetics, isolation & purification)
  • Receptors, Antigen, T-Cell, alpha-beta (chemistry, genetics, immunology)
  • Specific Pathogen-Free Organisms

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: