Quantitative analysis of changes in salivary mutans streptococci after orthodontic treatment.

Abstract

INTRODUCTION:

The purpose of this study was to analyze the initial changes in salivary mutans streptococci levels after orthodontic treatment with fixed appliances.

METHODS:

Our subjects consisted of 58 adults. Whole saliva and simplified oral hygiene index values were obtained at 4 time points: at debonding (T1), 1 week after debonding (T2), 5 weeks after debonding (T3), and 13 weeks after debonding (T4). Repeated measures analysis of variance was used to determine the time-related differences in salivary bacterial levels and the simplified oral hygiene index values among the 4 time points after quantifying the salivary levels of Streptococcus mutans, Streptococcus sobrinus, and total bacteria with real-time polymerase chain reaction.

RESULTS:

Simplified oral hygiene index values and total bacteria significantly decreased, but salivary mutans streptococci levels significantly increased after orthodontic treatment. The amounts of total bacteria in saliva significantly decreased at T3 (T1, T2 > T3, T4), and the simplified oral hygiene index values decreased at T2 (T1 > T2, T3, T4). However, salivary S mutans and S sobrinus significantly increased at T3 and T4, respectively (T1, T2 < T3 < T4). Furthermore, the proportion of mutans streptococci to total bacteria significantly increased at T4 (T1, T2, T3 < T4).

CONCLUSIONS:

This study suggests that careful hygienic procedures are needed to reduce the risk for dental caries after orthodontic treatment, despite overall improved oral hygiene status.

• Authors: Woo-Sun Jung, Ho Kim, So-Yoon Park, Eun-Jung Cho, Sug-Joon Ahn
• Journal: American journal of orthodontics and dentofacial orthopedics : official publication of the American Association of Orthodontists, its constituent societies, and the American Board of Orthodontics (Am J Orthod Dentofacial Orthop) Vol. 145 Issue 5 Pg. 603-9 (May 2014) ISSN: 1097-6752 [Electronic] United States
• PMID: 24785924 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 American Association of Orthodontists. Published by Mosby, Inc. All rights reserved.

Chemical References

• DNA, Bacterial

Topics

• Adult
• Bacterial Load
• DNA, Bacterial (analysis)
• Dental Debonding (methods)
• Female
• Follow-Up Studies
• Humans
• Male
• Oral Hygiene Index
• Orthodontic Brackets (microbiology)
• Orthodontic Retainers (microbiology)
• Orthodontics, Corrective
• Prospective Studies
• Real-Time Polymerase Chain Reaction (methods)
• Saliva (microbiology)
• Streptococcus mutans (classification, isolation & purification)
• Streptococcus sobrinus (classification, isolation & purification)
• Young Adult