Abstract |
Sclerostin is a negative regulator of bone formation. Sclerostin monoclonal antibody (Scl-Ab) treatment promoted bone healing in various animal models. To further evaluate the healing efficiency of Scl-Ab in osteotomy healing, we investigated the time course effects of systemic administration of Scl-Ab on fracture repair in rat femoral osteotomy model. A total of 120 six-month-old male SD rats were subjected to transverse osteotomy at the right femur mid-shaft. Rats were treated with vehicle or Scl-Ab treatment for 3, 6, or 9 weeks. Fracture healing was evaluated by radiography, micro-CT, micro-CT based angiography, 4-point bending mechanical test and histological assessment. Scl-Ab treatment resulted in significantly higher total mineralized callus volume fraction, BMD and enhanced neovascularization. Histologically, Scl-Ab treatment resulted in a significant reduction in fracture callus cartilage at week 6 and increase in bone volume at week 9, associated with a greater proportion of newly formed bone area at week 6 and 9 by fluorescence microscopy. Mechanical testing showed significantly higher ultimate load in Scl-Ab treatment group at week 6 and 9. This study has demonstrated that Scl-Ab treatment enhanced bone healing in a rat femoral osteotomy model, as reflected in increased bone formation, bone mass and bone strength.
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Authors | Pui Kit Suen, Yi-Xin He, Dick Ho Kiu Chow, Le Huang, Chaoyang Li, Hua Zhu Ke, Michael S Ominsky, Ling Qin |
Journal | Journal of orthopaedic research : official publication of the Orthopaedic Research Society
(J Orthop Res)
Vol. 32
Issue 8
Pg. 997-1005
(Aug 2014)
ISSN: 1554-527X [Electronic] United States |
PMID | 24782158
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2014 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. |
Chemical References |
- Antibodies, Monoclonal
- Bone Morphogenetic Proteins
- Genetic Markers
- Sost protein, rat
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Topics |
- Animals
- Antibodies, Monoclonal
(therapeutic use)
- Bone Morphogenetic Proteins
(immunology)
- Bony Callus
(drug effects)
- Femoral Fractures
(diagnostic imaging, pathology)
- Femur
(drug effects, pathology)
- Fracture Healing
(drug effects)
- Genetic Markers
(immunology)
- Male
- Neovascularization, Physiologic
(drug effects)
- Osteotomy
- Rats
- X-Ray Microtomography
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