HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Intestinal anti-inflammatory effects of oligosaccharides derived from lactulose in the trinitrobenzenesulfonic acid model of rat colitis.

Abstract
Intestinal microbiota modulation is becoming an interesting approach to manage inflammatory bowel disease and can be achieved by the administration of prebiotics. Previous studies showed the intestinal anti-inflammatory effects of the prebiotic lactulose. The aim of the present study was to test the preventative effects of oligosaccharides derived from lactulose with prebiotic properties (OsLu) in the trinitrobenzenesulfonic acid model of rat colitis and compare them with those of lactulose. Both treatments modified bacterial profile in intestinal contents, increasing the bifidobacteria and lactobacilli counts and up-regulating the production of short-chain fatty acids, although OsLu generated a larger amount. OsLu also inhibited to a greater extent different pro-inflammatory markers such as interleukins (IL) 1, 6, 12, and 23 and chemokines (MCP-1 and CINC-1). However, both prebiotics equally restored colonic epithelial integrity, evaluated both with a histological score (OsLu, 9.8 ± 2.2; and lactulose, 12.1 ± 2.1, vs colitic control, 27.3 ± 3.3) and by measuring several key proteins of the mucosal barrier (MUC-2, MUC-3, and TTF-3). OsLu effect was also associated with an inhibition of iNOS expression and a reduction of Th17 cell activity in the inflamed tissue that facilitated the intestinal mucosa barrier recovery. In conclusion, OsLu showed a better anti-inflammatory profile than lactulose in this model of experimental colitis.
AuthorsFrancesca Algieri, Alba Rodríguez-Nogales, Natividad Garrido-Mesa, Teresa Vezza, José Garrido-Mesa, M Pilar Utrilla, Antonia Montilla, Alejandra Cardelle-Cobas, Agustín Olano, Nieves Corzo, Eduardo Guerra-Hernández, Antonio Zarzuelo, M Elena Rodriguez-Cabezas, Julio Galvez
JournalJournal of agricultural and food chemistry (J Agric Food Chem) Vol. 62 Issue 19 Pg. 4285-97 (May 14 2014) ISSN: 1520-5118 [Electronic] United States
PMID24766341 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Anti-Inflammatory Agents
  • Chemokines
  • Interleukins
  • Oligosaccharides
  • Prebiotics
  • Lactulose
  • Trinitrobenzenesulfonic Acid
Topics
  • Animals
  • Anti-Inflammatory Agents (administration & dosage, chemistry)
  • Chemokines (genetics, immunology)
  • Colitis (chemically induced, drug therapy, immunology, microbiology)
  • Disease Models, Animal
  • Female
  • Humans
  • Interleukins (genetics, immunology)
  • Intestines (microbiology)
  • Lactulose (administration & dosage, chemistry)
  • Microbiota (drug effects)
  • Oligosaccharides (administration & dosage, chemistry)
  • Prebiotics (analysis)
  • Rats
  • Rats, Wistar
  • Trinitrobenzenesulfonic Acid (adverse effects)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: