[Effects of chlorogenic acid on the viability and HIF-1alpha mRNA expression of PC12 cells exposed to hypoxia].

Abstract

OBJECTIVE:

To investigate the effects of chlorogenic acid on the viability and HIF-1alpha mRNA expression of PC12 cells exposed to hypoxia.

METHODS:

PC12 cells were cultured in trigas incubator in order to establish the hypoxic condition. The effects of chlorogenic acid on the cells were evaluated by morphological observation, cell viability and LDH release assays as well as the examination of mRNA expression level of HIF-1alpha.

RESULTS:

Chlorogenic acid significantly improved the viability of cells exposed to hypoxia, decreased LDH release, arrested the cell cycle on G1 phase, and increased the gene expression level of HIF-1alpha.

CONCLUSION:

Chlorogenic acid protects PC12 cells from hypoxic damage by improving the expression of HIF-1alpha.

• Authors: Xu Jia, Zhong-Xue Fu, Juan Yao, Hui-Ping Ma
• Journal: Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials (Zhong Yao Cai) Vol. 36 Issue 10 Pg. 1644-7 (Oct 2013) ISSN: 1001-4454 [Print] China
• PMID: 24761676 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Hif1a protein, rat
• Hypoxia-Inducible Factor 1, alpha Subunit
• Plant Extracts
• RNA, Messenger
• Chlorogenic Acid
• Lactate Dehydrogenases

Topics

• Animals
• Cell Cycle (drug effects)
• Cell Hypoxia
• Cell Proliferation (drug effects)
• Cell Survival (drug effects)
• Chlorogenic Acid (pharmacology)
• Gene Expression Regulation, Neoplastic (drug effects)
• Hypoxia-Inducible Factor 1, alpha Subunit (genetics, metabolism)
• Lactate Dehydrogenases (metabolism)
• PC12 Cells
• Plant Extracts (pharmacology)
• Plants, Medicinal (chemistry)
• RNA, Messenger (genetics, metabolism)
• Rats
• Real-Time Polymerase Chain Reaction