[Establishment of a rat model of subacute toxic encephalopathy induced by 1, 2-dichloroethane].

Abstract

OBJECTIVE:

To establish a rat model of 1,2-dichloroethane (DCE)-induced subacute toxic encephalopathy.

METHODS:

Sixty Sprague-Dawley rats were randomly divided into five groups: negative control, positive control, low-dose DCE (1 472 mg/m(3)), middle-dose DCE (2 550 mg/m(3)), and high-dose DCE (4 418 mg/m(3)). The three DCE groups received static inhalation of DCE 6 hours a day for 6 consecutive days. The positive control group received intraperitoneal injection of lipopolysaccharide (5 mg/kg) and were sacrificed 8 hours after injection. Blood and brain tissue were collected, followed by determination of brain water content and HE staining for pathological examination of brain tissue.

RESULTS:

The rats in DCE groups suffered decreased body weight with increasing DCE dose (P < 0.01), and brain water content rose with increasing DCEdose. The brain water content of middle-dose DCE group (80.09 ± 0.14%) and high-dose DCE group (80.28±0.10%) increased significantly as compared with that of the negative control group (79.46±0.23%) (P < 0.001). Optical microscopy discovered loose structure and vasodilation in the brain tissue of middle-dose DCE group, indicating obvious brain edema; the high-dose DCE group and positive control group had spongiform and vacuolated brain tissues with severe vascular dilation, indicating severe brain edema.

CONCLUSION:

A rat model of subacute toxic encephalopathy induced by 1, 2-dichloroethane has been successfully established.

• Authors: Yanjun Deng, Wei Zhu, Baxiong Wei, Xuan Zhou, Yiwei Su, Yuan Gao, Yimin Liu
• Journal: Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases (Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi) Vol. 32 Issue 4 Pg. 260-3 (Apr 2014) ISSN: 1001-9391 [Print] China
• PMID: 24754938 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Ethylene Dichlorides
• ethylene dichloride

Topics

• Animals
• Disease Models, Animal
• Ethylene Dichlorides (toxicity)
• Male
• Neurotoxicity Syndromes
• Rats
• Rats, Sprague-Dawley