Effect of trapidil in myocardial ischemia-reperfusion injury in rabbit.

Abstract

OBJECTIVES:

To evaluate the cardioprotective effects of trapidil on myocardial ischemia-reperfusion injury (MIRI) in rabbits.

MATERIALS AND METHODS:

Rabbits were subjected to 40 min of myocardial ischemia followed by 120 min of reperfusion. Blood for superoxide dismutase (SOD) and malondialdehyde (MDA) were estimated. At the end of reperfusion, the rabbits were sacrificed and the hearts were isolated for histological examination. An apoptotic index (AI) was determined using the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end-labeling (TUNEL) method. The expression of apoptosis-related proteins Bax and Bcl-2 was analyzed using immunohistochemistry. Statistical analyses were performed by one-way analysis of variance (ANOVA), P < 0.05 considered statistically significant.

RESULTS:

Trapidil caused a significant (P < 0.05) increase in SOD activity, as decreased MDA levels and significantly (P < 0.05) reduced the expression of Bax as compared with the ischemia-reperfusion (IR) control group.

CONCLUSION:

Trapidil may attenuate the myocardial damage produced by IR injury and offer potential cardioprotective action.

• Authors: Mingjie Liu, Qi Sun, Qiang Wang, Xiuying Wang, Peng Lin, Ming Yang, Yuanyuan Yan
• Journal: Indian journal of pharmacology (Indian J Pharmacol) 2014 Mar-Apr Vol. 46 Issue 2 Pg. 207-10 ISSN: 1998-3751 [Electronic] India
• PMID: 24741195 (Publication Type: Journal Article)

Chemical References

• Antioxidants
• Cardiotonic Agents
• Proto-Oncogene Proteins c-bcl-2
• bcl-2-Associated X Protein
• Malondialdehyde
• Superoxide Dismutase
• Trapidil

Topics

• Animals
• Antioxidants (metabolism)
• Apoptosis (drug effects)
• Cardiotonic Agents (administration & dosage, pharmacology, therapeutic use)
• Male
• Malondialdehyde (blood, metabolism)
• Microscopy, Electron, Transmission
• Myocardial Reperfusion Injury (metabolism, pathology, prevention & control)
• Myocardium (metabolism, ultrastructure)
• Proto-Oncogene Proteins c-bcl-2 (metabolism)
• Rabbits
• Superoxide Dismutase (blood, metabolism)
• Trapidil (administration & dosage, pharmacology, therapeutic use)
• bcl-2-Associated X Protein (metabolism)