Abstract | BACKGROUND: Mycobacterium ulcerans (M. ulcerans) causes a devastating necrotising infection of skin tissue leading to progressive ulceration. M. ulcerans is the only human pathogen that secretes mycolactone, a polyketide molecule with potent cytotoxic and immunomodulatory properties. These unique features make mycolactone an attractive biomarker for M. ulcerans disease. We sought to measure the concentration of mycolactone within lesions of patients with Buruli ulcer before, during and after antibiotic treatment to evaluate its association with the clinical and bacteriological response to therapy. METHODS: Biopsies of M. ulcerans infected skin lesions were obtained from patients before, during and after antibiotic therapy. Lipids were extracted from the biopsies and concentration of mycolactone was assayed by mass spectrometry and a cytotoxicity assay and correlated with clinical and bacteriological response to therapy. RESULTS: Baseline concentration of mycolactone measured by mass spectrometry predicted time to complete healing of small nodules and ulcers. Even though intra-lesional concentrations of mycolactone declined with antibiotic treatment, the toxin was still present after antibiotic treatment for 6 weeks and also 4 weeks after the end of treatment for 8 weeks in a subgroup of patients with slowly healing lesions. Additionally viable bacilli were detected in a proportion of these slowly healing lesions during and after treatment. CONCLUSIONS:
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Authors | Fred S Sarfo, Richard O Phillips, Jihui Zhang, Mohammed K Abass, Justice Abotsi, Yaw A Amoako, Yaw Adu-Sarkodie, Clive Robinson, Mark H Wansbrough-Jones |
Journal | BMC infectious diseases
(BMC Infect Dis)
Vol. 14
Pg. 202
(Apr 15 2014)
ISSN: 1471-2334 [Electronic] England |
PMID | 24731247
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Anti-Bacterial Agents
- Macrolides
- mycolactone
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Topics |
- Adolescent
- Adult
- Aged
- Animals
- Anti-Bacterial Agents
(pharmacokinetics, therapeutic use)
- Buruli Ulcer
(drug therapy, metabolism)
- Child
- Child, Preschool
- Female
- Humans
- Macrolides
(pharmacokinetics, therapeutic use)
- Male
- Mass Spectrometry
- Mice
- Middle Aged
- Mycobacterium ulcerans
(isolation & purification)
- Skin
(chemistry, metabolism)
- Subcutaneous Tissue
(chemistry, metabolism)
- Tissue Distribution
- Young Adult
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