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Antigen-specific over-expression of human cartilage glycoprotein 39 on CD4+ CD25+ forkhead box protein 3+ regulatory T cells in the generation of glucose-6-phosphate isomerase-induced arthritis.

Abstract
Human cartilage gp-39 (HC gp-39) is a well-known autoantigen in rheumatoid arthritis (RA). However, the exact localization, fluctuation and function of HC gp-39 in RA are unknown. Therefore, using a glucose-6-phosphate isomerase (GPI)-induced model of arthritis, we investigated these aspects of HC gp-39 in arthritis. The rise in serum HC gp-39 levels was detected on the early phase of GPI-induced arthritis (day 7) and the HC gp-39 mRNA was increased significantly on splenic CD4(+) T cells on day7, but not on CD11b(+) cells. Moreover, to identify the characterization of HC gp-39(+) CD4(+) T cells, we assessed the analysis of T helper (Th) subsets. As a result, HC gp-39 was expressed dominantly in CD4(+) CD25(+) forkhead box protein 3 (FoxP3)(+) refulatory T cells (T(reg)), but not in Th1, Th2 or Th17 cells. Furthermore, to investigate the effect of HC gp-39 to CD4(+) T cells, T cell proliferation assay and cytokine production from CD4(+) T cells using recombinant HC gp-39 was assessed. We found that GPI-specific T cell proliferation and interferon (IFN)-γ or interleukin (IL)-17 production were clearly suppressed by addition of recombinant HC gp-39. Antigen-specific over-expression of HC gp-39 in splenic CD4(+) CD25(+) FoxP3(+) T(reg) cells occurs in the induction phase of GPI-induced arthritis, and addition of recombinant HC gp-39 suppresses antigen-specific T-cell proliferation and cytokine production, suggesting that HC gp-39 in CD4(+) T cells might play a regulatory role in arthritis.
AuthorsY Tanaka, I Matsumoto, A Inoue, N Umeda, C Takai, T Sumida
JournalClinical and experimental immunology (Clin Exp Immunol) Vol. 177 Issue 2 Pg. 419-27 (Aug 2014) ISSN: 1365-2249 [Electronic] England
PMID24730590 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Copyright© 2014 British Society for Immunology.
Chemical References
  • Adipokines
  • CHI3L1 protein, human
  • Chitinase-3-Like Protein 1
  • Cytokines
  • Epitopes, T-Lymphocyte
  • Forkhead Transcription Factors
  • Foxp3 protein, mouse
  • Interleukin-2 Receptor alpha Subunit
  • Lectins
  • Glucose-6-Phosphate Isomerase
Topics
  • Adipokines (blood, genetics, metabolism)
  • Animals
  • Arthritis, Experimental (genetics, immunology, metabolism)
  • Chitinase-3-Like Protein 1
  • Cytokines (biosynthesis)
  • Epitopes, T-Lymphocyte (immunology)
  • Forkhead Transcription Factors (metabolism)
  • Gene Expression
  • Glucose-6-Phosphate Isomerase (adverse effects, immunology)
  • Humans
  • Immunophenotyping
  • Interleukin-2 Receptor alpha Subunit (metabolism)
  • Lectins (blood, genetics, metabolism)
  • Lymphocyte Activation (immunology)
  • Male
  • Mice
  • Phenotype
  • Protein Transport
  • Spleen (cytology, metabolism)
  • T-Lymphocyte Subsets (immunology, metabolism)
  • T-Lymphocytes, Regulatory (immunology, metabolism)

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