Abstract |
Human cartilage gp-39 (HC gp-39) is a well-known autoantigen in rheumatoid arthritis (RA). However, the exact localization, fluctuation and function of HC gp-39 in RA are unknown. Therefore, using a glucose-6-phosphate isomerase (GPI)-induced model of arthritis, we investigated these aspects of HC gp-39 in arthritis. The rise in serum HC gp-39 levels was detected on the early phase of GPI-induced arthritis (day 7) and the HC gp-39 mRNA was increased significantly on splenic CD4(+) T cells on day7, but not on CD11b(+) cells. Moreover, to identify the characterization of HC gp-39(+) CD4(+) T cells, we assessed the analysis of T helper (Th) subsets. As a result, HC gp-39 was expressed dominantly in CD4(+) CD25(+) forkhead box protein 3 (FoxP3)(+) refulatory T cells (T(reg)), but not in Th1, Th2 or Th17 cells. Furthermore, to investigate the effect of HC gp-39 to CD4(+) T cells, T cell proliferation assay and cytokine production from CD4(+) T cells using recombinant HC gp-39 was assessed. We found that GPI-specific T cell proliferation and interferon (IFN)-γ or interleukin (IL)-17 production were clearly suppressed by addition of recombinant HC gp-39. Antigen-specific over-expression of HC gp-39 in splenic CD4(+) CD25(+) FoxP3(+) T(reg) cells occurs in the induction phase of GPI-induced arthritis, and addition of recombinant HC gp-39 suppresses antigen-specific T-cell proliferation and cytokine production, suggesting that HC gp-39 in CD4(+) T cells might play a regulatory role in arthritis.
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Authors | Y Tanaka, I Matsumoto, A Inoue, N Umeda, C Takai, T Sumida |
Journal | Clinical and experimental immunology
(Clin Exp Immunol)
Vol. 177
Issue 2
Pg. 419-27
(Aug 2014)
ISSN: 1365-2249 [Electronic] England |
PMID | 24730590
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2014 British Society for Immunology. |
Chemical References |
- Adipokines
- CHI3L1 protein, human
- Chitinase-3-Like Protein 1
- Cytokines
- Epitopes, T-Lymphocyte
- Forkhead Transcription Factors
- Foxp3 protein, mouse
- Interleukin-2 Receptor alpha Subunit
- Lectins
- Glucose-6-Phosphate Isomerase
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Topics |
- Adipokines
(blood, genetics, metabolism)
- Animals
- Arthritis, Experimental
(genetics, immunology, metabolism)
- Chitinase-3-Like Protein 1
- Cytokines
(biosynthesis)
- Epitopes, T-Lymphocyte
(immunology)
- Forkhead Transcription Factors
(metabolism)
- Gene Expression
- Glucose-6-Phosphate Isomerase
(adverse effects, immunology)
- Humans
- Immunophenotyping
- Interleukin-2 Receptor alpha Subunit
(metabolism)
- Lectins
(blood, genetics, metabolism)
- Lymphocyte Activation
(immunology)
- Male
- Mice
- Phenotype
- Protein Transport
- Spleen
(cytology, metabolism)
- T-Lymphocyte Subsets
(immunology, metabolism)
- T-Lymphocytes, Regulatory
(immunology, metabolism)
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