A novel vaccine against Porcine circovirus type 2 (PCV2) and Streptococcus equi ssp. zooepidemicus (SEZ) co-infection.

Abstract

To develop a vaccine against Porcine circovirus type 2 (PCV2) and Streptococcus equi ssp. zooepidemicus (SEZ) co-infection, the genes of porcine IL-18, capsid protein (Cap) of PCV2 and M-like protein (SzP) of SEZ were inserted into the swinepox virus (SPV) genome by homologous recombination. The recombinant swinepox virus rSPV-ICS was verified by PCR and indirect immunofluorescence assays. To evaluate the immunogenicity of rSPV-ICS, 28 PCV2 and SEZ seronegative Bama minipigs were immunized with rSPV-ICS (n=8), commercial PCV2 vaccine and SEZ vaccine (n=8) or wild type SPV (n=8). The results showed that SzP-specific antibody and PCV2 neutralizing antibody of the rSPV-ICS immunized group increased significantly compared to the wild type SPV treated group after vaccination and increased continuously over time. The levels of IL-4 and IFN-γ in the rSPV-ICS immunized group were significantly higher than the other three groups, respectively. After been co-challenged with PCV2 and SEZ, 87.5% piglets in rSPV-ICS immunized group were survived. Significant reductions in gross lung lesion score, histopathological lung lesion score, and lymph node lesion score were noticed in the rSPV-ICS immunized group compared with the wtSPV treated group. The results suggested that the recombinant rSPV-ICS provided piglets with significant protection against PCV2-SEZ co-infection; thus, it offers proof-of-principle for the development of a vaccine for the prevention of these swine diseases.

• Authors: Hui-xing Lin, Zhe Ma, Xu-qiu Yang, Hong-jie Fan, Cheng-ping Lu
• Journal: Veterinary microbiology (Vet Microbiol) Vol. 171 Issue 1-2 Pg. 198-205 (Jun 25 2014) ISSN: 1873-2542 [Electronic] Netherlands
• PMID: 24726504 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier B.V. All rights reserved.

Chemical References

• Antibodies, Neutralizing
• Antibodies, Viral
• Bacterial Proteins
• Bacterial Vaccines
• Vaccines, Synthetic
• Viral Proteins
• Viral Vaccines

Topics

• Animals
• Antibodies, Neutralizing (blood)
• Antibodies, Viral (blood)
• Bacterial Proteins (genetics, immunology)
• Bacterial Vaccines (immunology, standards)
• Circoviridae Infections (complications, pathology, prevention & control, veterinary)
• Circovirus (genetics, immunology)
• Coinfection
• Immunization (veterinary)
• Streptococcal Infections (complications, pathology, prevention & control, veterinary)
• Streptococcus equi (genetics, immunology)
• Suipoxvirus (genetics)
• Swine
• Swine Diseases (prevention & control)
• Vaccines, Synthetic (genetics, immunology)
• Viral Proteins (genetics)
• Viral Vaccines (immunology, standards)