37-kDa
laminin receptor precursor (37LRP) has a crucial role in migration of some human
cancers. Epithelial-to-mesenchymal transition (EMT) has received much attention in invasion and
metastasis of
lung cancer. Nevertheless, the role of 37LRP is not entirely clear in EMT promotion of
lung cancer at present. In this study, we firstly examined the possible role of 37LRP in the invasiveness and
metastasis process of
lung cancer using immunohistochemistry of 80
lung adenocarcinoma cases, western blot and real-time PCR of 12 fresh
lung adenocarcinoma tissues. The results showed that 37LRP significantly correlated with clinical stage and were highly expressed in metastatic
lung adenocarcinomas compared with nonmetastatic ones. In vitro, we observed that 37LRP significantly increased the adhesive, invasive and metastatic abilities of human
lung adenocarcinoma cell lines A549 by 37LRP-lentivirus interference. Furthermore, inoculation of A549 cells transduced with 37LRP-lentivirus in nude mice resulted in multi-
metastases including the lung. In addition, western blotting and immunofluorescence were used to detect the significant difference in expression of
E-cadherin and
fibronectin in A549 by 37LRP-lentivirus interference compared with 37LRP-small interference RNA-lentivirus interference in vitro and vivo. The data indicated that A549 cells of epithelial cell characteristics might be induced to undergo EMT by 37LRP. A549 cells transduced with 37LRP-lentivirus showed marked morphological changes, accompanied by the decrease of epithelial marker
E-cadherin and the increase of mesenchymal marker
fibronectin. These results indicated that 37LRP may promote
lung adenocarcinoma invasion and
metastasis via the mechanism of EMT.