Abstract | OBJECTIVE: MATERIALS AND METHODS: In vitro bactericidal properties of GTE were analysed by adding GTE to a suspension of uropathogenic E. coli (UPEC), streaking on MacConkey agar, and incubating overnight. In vivo effects of intravesical instillation of GTE on bacterial cystitis was analysed using a rat model of bacterial cystitis. In all, 42 female Sabra rats weighing 200-260 g were divided into five groups. Parameters measured were bladder weight (percentage of the total rat weight), dipstick urine analysis and histopathological changes in the bladder. Histological changes evaluated were degree of oedema, mixed inflammatory infiltration, urothelial epithelial invasion by neutrophils and reactive atypia. RESULTS: CONCLUSIONS:
Intravesical instillation of GTE attenuated the inflammatory response to UPEC-SR71-induced bacterial cystitis and is a novel approach to the treatment of bacterial cystitis. High concentrations of intravesical GTE did not cause histologically evident damage to the rat bladder. The results of this study are preliminary and further studies will be needed to explore the feasibility of using this approach in humans.
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Authors | Shilo Rosenberg, Ray Horowitz, Shunit Coppenhagen-Glazer, Galina Pizov, Anna Elia, Ofer N Gofrit, Isaac Ginsburg, Dov Pode |
Journal | BJU international
(BJU Int)
Vol. 114
Issue 4
Pg. 601-7
(Oct 2014)
ISSN: 1464-410X [Electronic] England |
PMID | 24712880
(Publication Type: Journal Article)
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Copyright | © 2013 The Authors. BJU International © 2013 BJU International. |
Chemical References |
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Topics |
- Administration, Intravesical
- Animals
- Camellia sinensis
- Cystitis
(drug therapy, microbiology)
- Disease Models, Animal
- Escherichia coli Infections
(drug therapy)
- Female
- Phytotherapy
- Plant Extracts
(administration & dosage)
- Plant Leaves
- Rats
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