Anti-inflammatory effects of triptolide in LPS-induced acute lung injury in mice.

Triptolide is one of the main active components of Chinese herb Tripterygium wilfordii Hook F, which has been demonstrated to have anti-inflammatory properties. The aim of this study was to investigate the effects of triptolide on lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice and to clarify the possible mechanisms. Mice were administered intranasally with LPS to induce lung injury. Triptolide was administered intraperitoneally 1 h before LPS challenge. Triptolide-treated mice exhibited significantly reduced leukocyte, myeloperoxidase (MPO) activity, edema of the lung, as well as TNF-α, IL-1β, and IL-6 production in the bronchoalveolar lavage fluid compared with LPS-treated mice. Additionally, Western blot analysis showed that triptolide inhibited the phosphorylation of inhibitor-kappa B kinase-alpha (IκB-α), p65, nuclear factor kappa B (NF-κB), p38, extracellular receptor kinase (ERK), and Jun N-terminal kinase (JNK) and the expression of Toll-like receptor 4 (TLR4) caused by LPS. In conclusion, our results suggested that the promising anti-inflammatory mechanism of triptolide may be that triptolide activates peroxisome proliferation-activated receptor gamma (PPAR-γ), thereby attenuating an LPS-induced inflammatory response. Triptolide may be a promising potential therapeutic reagent for ALI treatment.
AuthorsDong Wei, Zhihong Huang
JournalInflammation (Inflammation) Vol. 37 Issue 4 Pg. 1307-16 (Aug 2014) ISSN: 1573-2576 [Electronic] United States
PMID24706025 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Anti-Inflammatory Agents
  • Diterpenes
  • Epoxy Compounds
  • Interleukin-1beta
  • Interleukin-6
  • Ligands
  • Lipopolysaccharides
  • NF-kappa B
  • PPAR gamma
  • Phenanthrenes
  • Plant Extracts
  • Tumor Necrosis Factor-alpha
  • triptolide
  • Peroxidase
  • Acute Lung Injury (drug therapy)
  • Animals
  • Anti-Inflammatory Agents (pharmacology)
  • Diterpenes (pharmacology)
  • Epoxy Compounds (pharmacology)
  • Interleukin-1beta (metabolism)
  • Interleukin-6 (metabolism)
  • Leukocytes (cytology)
  • Ligands
  • Lipopolysaccharides (chemistry)
  • MAP Kinase Signaling System
  • Male
  • Mice
  • Mice, Inbred BALB C
  • NF-kappa B (metabolism)
  • PPAR gamma (metabolism)
  • Peroxidase (metabolism)
  • Phenanthrenes (pharmacology)
  • Plant Extracts (chemistry)
  • Tripterygium (metabolism)
  • Tumor Necrosis Factor-alpha (metabolism)

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