Abstract | BACKGROUND & AIMS: METHODS: Intestinal tissues were isolated from patients undergoing routine endoscopy or from patients undergoing intestinal surgery for colon cancer or IBD; healthy surrounding tissues were collected as controls. Isolated lamina propria mononuclear cells (LPMCs) and mucosal tissue explants were incubated with otelixizumab for 24 or 48 hours. Production of inflammatory cytokines was determined by enzyme-linked immunosorbent assay. Levels of 36 cytokines and chemokines and phosphorylation of 39 receptor tyrosine kinases and signaling molecules were measured using protein arrays. Immunoblot analysis was used to analyze T-cell transcription factors. RESULTS: CONCLUSIONS: We observed anti-inflammatory effects of anti-CD3 in inflamed intestinal tissues from patients with IBD. The antibody appears to down-regulate T-cell activation via IL-10.
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Authors | Anna Vossenkämper, Christian Hundsrucker, Kevin Page, André van Maurik, Theodore J Sanders, Andrew J Stagg, Lisa Das, Thomas T MacDonald |
Journal | Gastroenterology
(Gastroenterology)
Vol. 147
Issue 1
Pg. 172-83
(Jul 2014)
ISSN: 1528-0012 [Electronic] United States |
PMID | 24704524
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2014 AGA Institute. Published by Elsevier Inc. All rights reserved. |
Chemical References |
- Antibodies, Monoclonal
- Antibodies, Monoclonal, Humanized
- CD3 Complex
- Cytokines
- Interleukin-17
- Phosphoproteins
- Interleukin-10
- Interferon-gamma
- otelixizumab
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Topics |
- Adolescent
- Adult
- Antibodies, Monoclonal
(pharmacology)
- Antibodies, Monoclonal, Humanized
(pharmacology)
- Biopsy
- CD3 Complex
(immunology)
- Case-Control Studies
- Colon
(drug effects, metabolism, pathology)
- Cytokines
(metabolism)
- Female
- Humans
- Immune Tolerance
(drug effects)
- Inflammatory Bowel Diseases
(metabolism, pathology)
- Interferon-gamma
(metabolism)
- Interleukin-10
(metabolism)
- Interleukin-17
(metabolism)
- Intestinal Mucosa
(drug effects, metabolism, pathology)
- Male
- Middle Aged
- Phosphoproteins
(metabolism)
- Young Adult
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