Efficacy of a novel topical fipronil, (S)-methoprene, eprinomectin and praziquantel combination against naturally acquired intestinal nematode and cestode infections in cats.

Abstract

The efficacy of a novel topical combination formulation of fipronil, (S)-methoprene, eprinomectin and praziquantel against naturally acquired intestinal nematode and cestode infections in cats was evaluated in seven negative control, blinded studies. Cats were selected based on a pre-treatment faecal examination indicating a patent infection with at least hookworms (two studies), Toxocara ascarids (one study), taeniid cestodes (two studies) or Dipylidium cestodes (two studies). In each study, cats were assigned randomly to blocks of two animals each, based on decreasing pre-treatment body weight and were randomly allocated to one of two groups of six to 12 cats: untreated (control) or treated with topical fipronil (8.3%, w/v), (S)-methoprene (10%, w/v), eprinomectin (0.4%, w/v) and praziquantel (8.3%, w/v) (BROADLINE(®), Merial) at 0.12 mL/kg body weight (providing a minimum of 10mg fipronil+12 mg S-methoprene+0.5mg eprinomectin+10mg praziquantel per kg body weight). The topical treatment was administered directly on the skin in the midline of the neck in a single spot once on Day 0. For parasite recovery and count, cats were euthanized humanely and necropsied seven or ten days after treatment. A single treatment with the novel topical combination product provided 91% efficacy against Ancylostoma braziliense, ≥ 99% efficacy against Ancylostoma tubaeforme, and >97% efficacy against Toxocara cati. Similarly, excellent efficacy was established against Taenia taeniaeformis, Dipylidium caninum and Diplopylidium spp. as demonstrated by >97% and up to 100% reductions of cestode counts in the treated cats when compared to the untreated controls (P<0.01). All cats accepted the treatment well based on health observations post-treatment and daily health observations. No adverse experiences or other health problems were observed throughout the studies. The results of this series of controlled studies demonstrated high efficacy and excellent acceptability of the novel topical combination formulation of fipronil, (S)-methoprene, eprinomectin and praziquantel against a broad range of feline intestinal nematode and cestode infections.

• Authors: Martin Knaus, Marawan A Abu-Madi, Froylán Ibarra-Velarde, Dawie J Kok, Ilir Kusi, Rezart Postoli, S Theodore Chester, Joseph Rosentel, Roberto Alva, Jennifer Irwin, Martin Visser, Renate Winter, Steffen Rehbein
• Journal: Veterinary parasitology (Vet Parasitol) Vol. 202 Issue 1-2 Pg. 18-25 (Apr 28 2014) ISSN: 1873-2550 [Electronic] Netherlands
• PMID: 24703071 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 The Authors. Published by Elsevier B.V. All rights reserved.

Chemical References

• Antiparasitic Agents
• Drug Combinations
• Pyrazoles
• Praziquantel
• Ivermectin
• eprinomectin
• Methoprene
• fipronil

Topics

• Animals
• Antiparasitic Agents (administration & dosage)
• Cat Diseases (drug therapy)
• Cats
• Cestoda
• Cestode Infections (drug therapy, veterinary)
• Drug Combinations
• Female
• Intestinal Diseases, Parasitic (drug therapy, veterinary)
• Ivermectin (administration & dosage, analogs & derivatives)
• Male
• Methoprene (administration & dosage)
• Nematoda
• Nematode Infections (drug therapy, veterinary)
• Praziquantel (administration & dosage)
• Pyrazoles (administration & dosage)
• Random Allocation
• Treatment Outcome