Two novel clerodane diterpenenes with NGF-potentiating activities from the twigs of Croton yanhuii.

Abstract

Nerve growth factor (NGF) and analog reagents to promote the neurite outgrowth of nerve cells against the neuron degeneration are expected to be potentially useful for the medical treatment of Alzheimer's disease. In our focus on the discovery of bioactive diterpenes, we investigated the chemical constituents of the plant Croton yanhuii. This investigation led to the isolation and identification of two novel clerodane diterpenes (1 and 2). Their structures were elucidated on the basis of extensive 1D and 2D NMR (COSY, HMQC, HMBC, and NOESY) and mass (ESIMS and HR-ESIMS) spectroscopic data analyses. Further biological screenings showed that both of the compounds enhanced NGF-mediated neurite outgrowth from PC12 cells.

• Authors: Yihang Sun, Meicheng Wang, Quanhui Ren, Shen Li, Jing Xu, Yasushi Ohizumi, Chunfeng Xie, Da-Qing Jin, Yuanqiang Guo
• Journal: Fitoterapia (Fitoterapia) Vol. 95 Pg. 229-33 (Jun 2014) ISSN: 1873-6971 [Electronic] Netherlands
• PMID: 24685507 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier B.V. All rights reserved.

Chemical References

• Diterpenes, Clerodane
• crotonpene A
• crotonpene B
• Nerve Growth Factor

Topics

• Alzheimer Disease (drug therapy)
• Animals
• Croton (chemistry)
• Diterpenes, Clerodane (chemistry, isolation & purification, pharmacology, therapeutic use)
• Drug Synergism
• Magnetic Resonance Spectroscopy
• Molecular Structure
• Nerve Growth Factor (metabolism)
• Neurites (drug effects)
• PC12 Cells
• Plants, Medicinal
• Rats